Merck
All Photos(1)

1.08218

Supelco

Trifluoroacetic acid solution

(25% solution in water) for protein sequenation

Synonym(s):
Trifluoroacetic acid solution, TFA solution, TFA
Linear Formula:
CF3COOH
CAS Number:
Molecular Weight:
114.02
Beilstein:
5446934
MDL number:

Quality Level

form

liquid

composition

Water, 74.5-75.5% Karl Fischer

concentration

24.5-25.5% (trifluoroacetic acid)

density

1.12 g/cm3 at 20 °C

storage temp.

15-25°C

SMILES string

OC(=O)C(F)(F)F

InChI

1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

DTQVDTLACAAQTR-UHFFFAOYSA-N

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This Item
2995371.0817891707
Trifluoroacetic acid ≥99%, for protein sequencing

Sigma-Aldrich

299537

Trifluoroacetic acid

Trifluoroacetic acid for protein sequence analysis

Supelco

1.08178

Trifluoroacetic acid

Trifluoroacetic acid puriss. p.a., suitable for HPLC, ≥99.0% (GC)

Sigma-Aldrich

91707

Trifluoroacetic acid

form

liquid

form

liquid

form

liquid

form

liquid

concentration

24.5-25.5% (trifluoroacetic acid)

concentration

-

concentration

-

concentration

-

density

1.12 g/cm3 at 20 °C

density

1.489 g/mL at 20 °C (lit.)

density

1.489 g/mL at 20 °C (lit.)

density

1.489 g/mL at 20 °C (lit.)

storage temp.

15-25°C

storage temp.

-

storage temp.

2-30°C

storage temp.

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

composition

Water, 74.5-75.5% Karl Fischer

composition

-

composition

-

composition

-

Application

Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).

Analysis Note

Assay (alkalimetric): 24.5 - 25.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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