GF439-M

HumanKine^® TGF beta 1, Human Recombinant Xeno-Free

• Synonym(s): TGFB, TGFb1, Transforming Growth Factor-β1
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Properties

• Assay: >95% (SDS-PAGE)
• Quality Level: 100
• manufacturer/tradename: HumanKine^®
• impurities: <0.1 μg/mg protein endotoxin (1 EU/μg)
• UniProt accession no.: P01137

Description

General description

HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.

Transforming growth factor beta (TGF-B) is a multifunctional cytokine that controls proliferation, differentiation, and other functions in many cell types. TGF-B acts synergistically with TGF-A in inducing transformation. It also acts as a negative autocrine growth factor. TGF-B regulates the actions of many other growth factors and determines a positive or negative direction of their effects. It plays an important role in bone remodeling. It is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Physical form

Product is presented in 50mM NaOAc pH 4.5 and filtered through a 0.2 micron filter before lyophilization.

Preparation Note

HumanKine TGF beta1 is manufactured using all non-animal reagents and is considered Xeno-Free

Store at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.

Analysis Note

Bioactivity assay: Determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2). ED50 is typically 0.1-0.5 ng/ml.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1