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Merck

MDH-2N1

Millicell® Disposable Hemocytometer

Neubauer Improved ruling pattern, 2 chambers/slide, 10 µL loading volume, individually wrapped

Millicell® Disposable Hemocytometer

Synonym(s):

Optical grade plastic hemocytometer, disposable hemocytometer, Cell Culture

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About This Item

UNSPSC Code:
41161502
NACRES:
NC.56

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material

colorless plastic

sterility

non-sterile

feature

pyrogen free

packaging

pack of 50 (individually wrapped)

parameter

10 μL sample volume

technique(s)

cell counting: suitable

W × L × H

25 mm × 75 mm × 1.6 mm

chamber depth

0.1 mm

size

2 wells

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General description

The Millicell® disposable hemocytometer is used to manually count cells. It consists of a surface-patterned two enclosed chambers with two ports for sample injection. Count cells with the in the same way as you do with the Neubauer Improved counting chamber.

The Neubauer Improved counting chamber consists of 9 large squares, each measuring 1 x 1 mm. Each square has a total volume of 0. 1 mm3. The cells are distributed over 3 x 3 large squares, each with 1 mm edge length and with a surface area of 1 mm2.

With the Millicell® disposable hemocytometer, you inject 10 µL of sample, stained or unstained, into the desired chamber.

Application

cell counting, cell culture, hemocytometer

Features and Benefits

  • Quartz grade optical plastic
  • No cover slips
  • No washing

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Cell counting protocol using a hemocytometer for monitoring cell viability. Discover other cell counting tools such as the Scepter™ 3.0 Cell Counter.

Cell validation data using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.

Data for validated human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.

Data for validated non-human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.

Protocols

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

Related Content

Monitor your cell cultures with ease with the Millicell® DCI Digital Cell Imager. From confluency to cell count and morphology, the Millicell® DCI automates cell culture measurements for quicker, more consistent culturing.

Questions

1–2 of 2 Questions  
  1. Is it sterile? 

    1 answer
    1. As listed in the 'PROPERTIES' section, this product is non-sterile.

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  2. What is the ruling pattern used for the Product ?

    1 answer
    1. A complete description around the product and its ruling pattern can be found in the "User Guide - MDH-2N1" on the product detail webpage in the Documents section.: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/144/042/mdh-2n1-ms.pdf

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