95284

Water

for molecular biology, suitable for FISH

• Synonym(s): H₂O
• Linear Formula: H₂O
• CAS Number: 7732-18-5
• Molecular Weight: 18.02
• Beilstein: 2050024
• EC Number: 231-791-2
• MDL number: MFCD00011332
• UNSPSC Code: 12191602
• PubChem Substance ID: 57653665
• NACRES: NA.31
• Grade: Molecular Biology
• Technique(s): FISH: suitable
• Bp: 100 °C (lit.)

Properties

• Product Name: Water, Molecular Biology, sterile filtered
• grade: Molecular Biology
• Quality Level: 100
• sterility: sterile-filtered
• form: liquid
• quality: sterile filtered
• shelf life: limited shelf life, expiry date on the label
• technique(s): FISH: suitable
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• refractive index: n20/D 1.34 (lit.)
• pH: 5-7
• conductivity: 0.0-5 μS/cm at 25 °C
• bp: 100 °C (lit.)
• mp: 0 °C (lit.)
• density: 1.000 g/mL at 3.98 °C (lit.)
• cation traces: Ca: <10 ppb; Mg: <10 ppb
• SMILES string: O
• InChI: 1S/H2O/h1H2
• InChI key: XLYOFNOQVPJJNP-UHFFFAOYSA-N

Description

General description

Molecular biology grade water is sterile filtered and ready to use, free from DNase, RNase, phosphatase, and protease contamination.

Application

Water, sterile filtered has been used:

• for cloning and plasmid construction.[1]
• for restriction digestion of PCR amplicons in polysaccharide A (PSA) restriction method.[2]
• in RNA isolation.[3][4][5]
• fluorescence in situ hybridization (FISH).[6]
• to dilute the samples of CVF (cervicovaginal fluid) and menstrual extracts for testing specificity through reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of mRNA. [7]
• for DNA quantification using quantitative polymerase chain reaction (qPCR).[8]

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves