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Merck

A7979

Bovine Serum Albumin solution

35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:

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Product Name

Bovine Serum Albumin solution, 35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

biological source

bovine

description

endotoxin tested

sterility

sterile-filtered

product line

BioXtra

assay

33-37% total protein basis (UV)

form

liquid

origin

USA origin

concentration

35% in DPBS

technique(s)

blocking: suitable
cell culture | mammalian: suitable

impurities

≤1000 EU/mL Endotoxin level

pH

6.5-7.5

foreign activity

Mycoplasma Test by Barile On raw material, none detected

shipped in

ambient

storage temp.

2-8°C

Quality Level

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1 of 4

This Item
A9576A3156A8806
origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

assay

33-37% total protein basis (UV)

assay

28-32% total protein basis (UV)

assay

≥99% (agarose gel electrophoresis)

assay

≥96% (agarose gel electrophoresis)

technique(s)

blocking: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

400

Quality Level

300

Quality Level

200

Quality Level

300

form

liquid

form

liquid

form

lyophilized powder

form

lyophilized powder

Application

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Katharina Janssen et al.
Methods in molecular biology (Clifton, N.J.), 1994, 31-39 (2019-05-28)
To better understand and model neurological, in particular neurodegenerative diseases, human induced pluripotent stem cells (hiPSCs) offer a great source for generation of neural cells. We provide a protocol for the differentiation of hiPSc-derived astrocytes in vitro. This protocol not
Brett S Peterson et al.
Cell reports, 24(1), 209-223 (2018-07-05)
SIRT3 is a nicotinamide adenine dinucleotide (NAD+)-dependent mitochondrial protein deacetylase purported to influence metabolism through post-translational modification of metabolic enzymes. Fuel-stimulated insulin secretion, which involves mitochondrial metabolism, could be susceptible to SIRT3-mediated effects. We used CRISPR/Cas9 technology to manipulate SIRT3 expression in
Mohammed Samer Shaban et al.
Nature communications, 12(1), 5536-5536 (2021-09-22)
Coronaviruses (CoVs) are important human pathogens for which no specific treatment is available. Here, we provide evidence that pharmacological reprogramming of ER stress pathways can be exploited to suppress CoV replication. The ER stress inducer thapsigargin efficiently inhibits coronavirus (HCoV-229E
Gustavo A Gomez et al.
Developmental biology, 449(2), 99-106 (2019-03-04)
The developmental biology of neural crest cells in humans remains unexplored due to technical and ethical challenges. The use of pluripotent stem cells to model human neural crest development has gained momentum. We recently introduced a rapid chemically defined approach
Jonathan De Smedt et al.
Cell death & disease, 12(1), 84-84 (2021-01-16)
To date, there is no representative in vitro model for liver sinusoidal endothelial cells (LSECs), as primary LSECs dedifferentiate very fast in culture and no combination of cytokines or growth factors can induce an LSEC fate in (pluripotent stem cell

Articles

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

Questions

1–4 of 4 Questions  
  1. Is it suitable for biotin/streptavidin-mediated detection systems?

    1 answer
    1. This product is not tested for biotin levels or suitability in biotin/streptavidin-mediated detection systems.

      Helpful?

  2. IS IT FREE FROM CALCIUM AND MAGNESIUM?

    1 answer
    1. This product is prepared in Dulbeccos Phosphate Buffered Saline without calcium and magnesium.

      Helpful?

  3. What is the concentration of this product?

    1 answer
    1. As shown in the DESCRIPTION section of the Product Detail Page, the BSA concentration for Product A7979, is 35% w/v in DPBS.

      Helpful?

  4. Hi, We have used Bovine serum albumin (A6003) and prepared a 10% BSA solution in buffer. There are also several BSA solutions available, but I can't see any information what BSA solid that was used (Product/Cat number). Has any the same origin?

    1 answer
    1. Product A6003 is a fatty acid-free BSA powder.

      See the links below to review the fatty acid-free BSA solutions currently prepared from this material.
      A1595, 10% BSA Solution, Fatty acid-free, in DPBS
      https://www.sigmaaldrich.com/product/sigma/a1595

      A9205, 30% BSA Solution, Fatty acid-free, in DPBS
      https://www.sigmaaldrich.com/product/sigma/a9205

      Helpful?

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