Merck
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E5134

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

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Synonym(s):
Sequestrene Na₂, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2
Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
CAS Number:
Molecular Weight:
372.24
Beilstein:
3900609
EC Number:
PubChem Substance ID:
NACRES:
NA.31

grade

for molecular biology

Assay

99.0-101.0% (titration)

form

powder

reaction suitability

reagent type: chelator

technique(s)

electrophoresis: suitable

impurities

≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

solubility

3  M NaOH: 1.6 g/10 mL

cation traces

Fe: ≤0.005%
Pb: ≤0.002%

foreign activity

DNase, RNase, NICKase and protease, none detected

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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1 of 4

This Item
E4884ED2SSE1644
assay

99.0-101.0% (titration)

assay

99.0-101.0%

assay

98.5-101.5% (titration)

assay

98.5-101.5%

form

powder

form

solid

form

powder

form

powder

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

impurities

≤0.005% Insolubles, ≤0.1% Nitrilotriacetic acid (NTA)

impurities

≤0.005% Insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

impurities

-

impurities

≤0.005% Phosphorus (P), ≤0.05% NH3, ≤0.1% Insoluble matter

mp

248 °C (dec.) (lit.)

mp

248 °C (dec.) (lit.)

mp

248 °C (dec.) (lit.)

mp

248 °C (dec.) (lit.)

General description

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests(3)

Application

EDTA disodium salt has been used in research science
  • as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more. (2)
  • an anticoagulant for whole blood collection (3)
  • a stabilizer solution for freezing cell culture samples (4)
  • in combination with PBS and trypsin, to trypsinize cells (5)
  • for phytoextraction of heavy metals in polluted soils (1)
Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Features and Benefits

  • Molecular Biology grade, suitable for many molecular biology applications
  • Free of endonucleases DNase, RNase, Nickase, and Protease

Pictograms

Exclamation markHealth hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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External quality assessment of methylmalonic acid and total homocysteine.
M?ller J, et al.
Clinical Chemistry, 45(9), 1536-1542 (1999)
Expression of ABCG2 and Bmi-1 in oral potentially malignant lesions and oral squamous cell carcinoma.
Dalley AJ, et al.
Cancer Medicine, 3(2), 273-283 (2014)
Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.
Ming-Kai Hsieh et al.
Scientific reports, 10(1), 10441-10441 (2020-06-28)
Screw loosening due to broken pedicles is a common complication resulting from the insertion of screws either with inadequate diameters or into an osteoporotic pedicle. In this novel in vitro study, we tried to clarify the contribution of the pedicle
Michi Miura et al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 28(3-4), 381-393 (2020-10-19)
CUT&RUN is a powerful tool to study protein-DNA interactions in vivo. DNA fragments cleaved by the targeted micrococcal nuclease identify the footprints of DNA-binding proteins on the chromatin. We performed CUT&RUN on human lung carcinoma cell line A549 maintained in

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