F3388

Ferric citrate

BioReagent, suitable for cell culture

• Synonym(s): Iron(III) citrate, Iron(III) citrate hydrate
• Empirical Formula (Hill Notation): C₆H₅FeO₇
• CAS Number: 3522-50-7
• Molecular Weight: 244.94
• EC Number: 222-536-6
• MDL number: MFCD00013098
• UNSPSC Code: 12352205
• PubChem Substance ID: 24894843
• NACRES: NA.75

Properties

• biological source: synthetic (organic)
• Quality Level: 300
• product line: BioReagent
• technique(s): cell culture | mammalian: suitable
• solubility: hot water: 0.01 g/mL, clear, orange-yellow
• shipped in: ambient
• storage temp.: room temp
• SMILES string: OC12CC(=O)O[Fe](OC(=O)C1)OC2=O
• InChI: 1S/C6H8O7.Fe/c7-3(8)1-6(13,5(11)12)2-4(9)10;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);/q;+3/p-3
• InChI key: NPFOYSMITVOQOS-UHFFFAOYSA-K

Description

Application

Ferric citrate provides a source of iron used in cell culture applications. It provides iron in a less toxic form than free iron salts. Ferric citrate has been used as medium supplement in human osteosarcoma cell (U2OS) culture,[1] human embryonic kidney (HEK293) cells[2] and adult primary cardiomyocytes.[3]

Biochem/physiol Actions

Ferric citrate is used as a component of protein-free medium. It is used as a substitute for transferrin in mouse hybridomas culture. Ferric citrate enhances IgM production.[4] It indirectly stimulates the mitogen-activated protein kinase (MAPK) by favoring high amphiregulin oncoprotein levels in colon cancer.[5]  Ferric citrate is effective in binding to phosphate in patients with dialysis-dependent chronic kidney disease.[6]

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves