F3422

Flt-3/Flk-2 ligand human

Name: Flt-3/Flk-2 ligand human
Brand: SIGMA

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥98% (SDS-PAGE and HPLC)

Synonym(s):

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About This Item

MDL number:

MFCD01093789

UNSPSC Code:

51111800

NACRES:

NA.32

Key Documents

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biological source

human

Quality Level

100

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE and HPLC)

form

lyophilized powder

potency

≤1.0 ng/mL

mol wt

17.6 kDa

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P36888

storage temp.

−20°C

Gene Information

human ... FLT3(2322)

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This Item	SRP3044	H5416	SRP3198
Sigma-Aldrich F3422 Flt-3/Flk-2 ligand human	Sigma-Aldrich SRP3044 Flt-3 Ligand human	Sigma-Aldrich H5416 FLT3 Ligand human	Sigma-Aldrich SRP3198 flt3-Ligand from mouse
biological source human	biological source human	biological source human	biological source mouse
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in HEK 293 cells	recombinant expressed in E. coli
assay ≥98% (SDS-PAGE and HPLC)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
Quality Level 100	Quality Level -	Quality Level 100	Quality Level -
form lyophilized powder	form lyophilized	form lyophilized powder	form lyophilized

General description

Mouse and human Flt-3 ligand share ~72% homology and both demonstrate cross-species activity.

Biochem/physiol Actions

Flt-3/Flk-2 Ligand (FL, or Flt-3L) is a transmembrane glycoprotein that binds and activates the Flt-3 receptor. Expression of Flt-3 is primarily restricted among hematopoietic cells to the most primitive progenitor cells.

Physical form

Lyophilized, 0.2 μm sterile filtered, with with 0.5x PBS.

Analysis Note

The biological activity is measured by its ability to stimulate the proliferation of human AML5 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Lyman, S., et al. et al.

The Cytokine Handbook, 727-727 (1998)

Characterization of the protein encoded by the flt3 (flk2) receptor-like tyrosine kinase gene.

S D Lyman et al.

Oncogene, 8(4), 815-822 (1993-04-01)

We have developed rabbit polyclonal antibodies to the C-terminus of the flt3-encoded protein, which is a member of the receptor tyrosine kinase family. Immunoprecipitation using this antiserum brings down two protein bands, a major band of 143 kDa and a

Effects of FLT3 ligand on proliferation and survival of myeloid leukemia cells.

H G Drexler et al.

Leukemia & lymphoma, 33(1-2), 83-91 (1999-04-08)

FLT3 ligand (FL) acting through its tyrosine kinase receptor FLT3 has pleiotropic and potent effects on hematopoietic cells. The well-described involvement of this ligand-receptor pair in physiological hematopoiesis raised the question whether FL and FLT3 also play a role in

Regulatory roles of the ligand for Flk2/Flt3 tyrosine kinase receptor on human hematopoiesis.

R Namikawa et al.

Stem cells (Dayton, Ohio), 14(4), 388-395 (1996-07-01)

The biological activities of the ligand for the Flk2/Flt3 receptor tyrosine kinase (FL) on human hematopoietic cells are reviewed. In in vitro studies, FL shows relatively few effects by itself on the proliferation and differentiation of hematopoietic cells, but exhibits

A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations.

W Matthews et al.

Cell, 65(7), 1143-1152 (1991-06-28)

To elucidate the molecular biology of the hematopoietic stem cell, we have begun to isolate genes from murine cell populations enriched in stem cell activity. One such cDNA encodes a novel receptor tyrosine kinase, designated fetal liver kinase-2 or flk-2

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