P1146

Phosphatase, Acid from potato

Name: Phosphatase, Acid from potato
Brand: SIGMA

lyophilized powder, ≥3.0 units/mg solid

Synonym(s):

(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato

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About This Item

CAS Number:

9001-77-8

EC Number:

3.1.3.2 (BRENDA, IUBMB)

EC Number:

232-630-9

MDL number:

MFCD00131847

UNSPSC Code:

12352204

eCl@ss:

42010102

NACRES:

NA.54

Key Documents

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form

lyophilized powder

Quality Level

300

specific activity

≥3.0 units/mg solid

mol wt

69 kDa

shipped in

wet ice

storage temp.

−20°C

SMILES string

O(CC(O)C)CC#C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

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This Item	SRE0048	P3752	10108227001
Sigma-Aldrich P1146 Phosphatase, Acid from potato	Sigma-Aldrich SRE0048 Phosphatase, Acid from potato	Sigma-Aldrich P3752 Phosphatase, Acid from potato	Roche 10108227001 Acid Phosphatase
specific activity ≥3.0 units/mg solid	specific activity 3.0-10.0 unit/mg solid	specific activity ≥0.5 unit/mg solid	specific activity ~2 units/mg protein (at 25 °C with 4-nitrophenyl phosphate as the substrate)
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. -
shipped in wet ice	shipped in wet ice	shipped in -	shipped in wet ice
mol wt 69 kDa	mol wt -	mol wt 69 kDa	mol wt -
Quality Level 300	Quality Level 400	Quality Level 200	Quality Level 100

General description

Acid phosphatase from potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points. [1]

Application

Acid phosphatase from potato has been used in a study to assess the potential allergenicity of novel gene products. [2] It has also been used in a study to remove eight phosphate groups from casein at a pH of 7.0. [3]

The activity of potato acid phosphatase in various surfactant medias was examined. Bis(2- ethylhexyl)sodium sulfosuccinate, Brij 35, and SDS at 3mM inhibited phosphatase activity while the surfactant cetyltrimethylammonium bromide enhanced activity. [4]

Biochem/physiol Actions

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Stability and activity of potato acid phosphatase in aqueous surfactant media.

J Lalitha et al.

Biochemistry and molecular biology international, 41(4), 797-803 (1997-04-01)

The effect of three different classes of surfactants viz., anionic, cationic and neutral on catalytic activity of potato acid phosphatase (AcPase) was studied. Anionic surfactants bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and sodium dodecyl sulfate (SDS) inhibited AcPase activity completely at 3

Potential allergenicity research of Cry1C protein from genetically modified rice.

Sishuo Cao et al.

Regulatory toxicology and pharmacology : RTP, 63(2), 181-187 (2012-04-17)

With the development of genetically modified crops, there has been a growing interest in available approaches to assess the potential allergenicity of novel gene products. We were not sure whether Cry1C could induce allergy. We examined the protein with three

Isolation and characterization of a homogeneous isoenzyme of wheat germ acid phosphatase.

P P Waymack et al.

Archives of biochemistry and biophysics, 288(2), 621-633 (1991-08-01)

An acid phosphatase (orthophosphoric monoester phosphohydrolase, acid optimum; EC 3.1.3.2) isoenzyme from wheat germ was purified 7000-fold to homogeneity. The effect of wheat germ sources and their relationship to the isoenzyme content and purification behavior of acid phosphatases was investigated.

Effects of multivalent cations on cell wall-associated Acid phosphatase activity.

S I Tu et al.

Plant physiology, 88(1), 61-68 (1988-09-01)

Primary cell walls, free from cytoplasmic contamination were prepared from corn (Zea mays L.) roots and potato (Solanum tuberosum) tubers. After EDTA treatment, the bound acid phosphatase activities were measured in the presence of various multivalent cations. Under the conditions

Purification, enzymatic properties, and active site environment of a novel manganese(III)-containing acid phosphatase.

Y Sugiura et al.

The Journal of biological chemistry, 256(20), 10664-10670 (1981-10-25)

A new manganese-containing acid phosphatase has been isolated and crystallized from sweet potato tubers. The pure enzyme contains one atom of manganese per Mr = 110,000 polypeptide and shows phosphatase activity toward various phosphate substrates. The pH optimum of the

View All Related Papers

Protocols

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

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