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W4502

Sigma-Aldrich

Water

Water

suitable for electrophoresis, PCR

Synonym(s):

DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water

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1 L
RUB 7,564.36
10 X 50 ML
RUB 14,284.24
6 X 1 L
RUB 37,192.93
10 L
RUB 73,038.29

RUB 7,564.36


Estimated to ship onJune 23, 2025Details


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1 L
RUB 7,564.36
10 X 50 ML
RUB 14,284.24
6 X 1 L
RUB 37,192.93
10 L
RUB 73,038.29

About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
MDL number:
UNSPSC Code:
12191602
PubChem Substance ID:
NACRES:
NA.31
technique(s):
PCR: suitable
electrophoresis: suitable
bp:
100 °C (lit.)
vapor pressure:
3 mmHg

RUB 7,564.36


Estimated to ship onJune 23, 2025Details


To order products, please contact your local dealer.

Product Name

Water, Nuclease-Free Water, for Molecular Biology

vapor density

<1 (vs air)

Quality Level

vapor pressure

3 mmHg

sterility

0.1 μm filtered

form

liquid

technique(s)

PCR: suitable
electrophoresis: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, RNase, Protease, free

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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Show Differences

1 of 4

This Item
9801-OP9528995284
Water Nuclease-Free Water, for Molecular Biology

W4502

Water

Water OmniPur&#174; Grade, Sterile, Nuclease Free

9801-OP

Water

Water for cell biology, sterile ultrafiltered

95289

Water

Water Molecular Biology, sterile filtered

95284

Water

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

technique(s)

PCR: suitable, electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

FISH: suitable

form

liquid

form

liquid

form

liquid

form

liquid

vapor density

<1 (vs air)

vapor density

-

vapor density

-

vapor density

-

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

mp

0 °C (lit.)

mp

0 °C (lit.)

mp

0 °C (lit.)

mp

0 °C (lit.)

Application

Water has been used-
  • in cDNA synthesis and PCR amplification[1][2]
  • for the dilution of rumen DNA sample prior to qPCR assay[3]
  • in PCR (polymerase chain reaction) and agarose gel electrophoresis[4]
  • for the elution of RNA during RNA isolation[5]

Features and Benefits

  • Nuclease-Free Water
  • DNase-Free, RNase-Free, Protease- Free
  • Not DEPC Treated
  • 0.1 um Filtered
  • Prepared from Double Distilled and Deionized Water
  • Suitable for use in Molecular Applications

This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).

Preparation Note

This product is prepared from double distilled and deionized water

Other Notes

Easily compare specifications for Water products with the Water specification table.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Reviews

Active Filters

  1. USA
    • Review 1
    • Vote 1
    1 out of 5 stars.

    This water has microbial contamination!

    We used this water (Lot #0000387784) to dilute DNA for cell culture, but the cells kept getting contaminated. Eventually we traced it back to the water. Even a freshly opened bottle had microbial growth after mixing with culture medium. This ended up ruining thousands of dollars of experiments.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  2. 1 Ratings-only review

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