W4502

Water

Nuclease-Free Water, for Molecular Biology

• Synonym(s): DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water
• Linear Formula: H₂O
• CAS Number: 7732-18-5
• Molecular Weight: 18.02
• Beilstein: 2050024
• EC Number: 231-791-2
• MDL number: MFCD00011332
• PubChem Substance ID: 24901995
• NACRES: NA.31

Properties

• vapor density: <1 (vs air)
• Quality Level: 200
• vapor pressure: 3 mmHg
• sterility: 0.1 μm filtered
• form: liquid
• technique(s): PCR: suitable; electrophoresis: suitable
• refractive index: n20/D 1.34 (lit.)
• pH: 5-7
• bp: 100 °C (lit.)
• mp: 0 °C (lit.)
• density: 1.000 g/mL at 3.98 °C (lit.)
• foreign activity: DNase, RNase, Protease, free
• SMILES string: O
• InChI: 1S/H2O/h1H2
• InChI key: XLYOFNOQVPJJNP-UHFFFAOYSA-N

Description

Application

Water has been used-

• in cDNA synthesis and PCR amplification
• for the dilution of rumen DNA sample prior to qPCR assay
• in PCR (polymerase chain reaction) and agarose gel electrophoresis
• for the elution of RNA during RNA isolation

Features and Benefits

• Nuclease-Free Water
• DNase-Free, RNase-Free, Protease- Free
• Not DEPC Treated
• 0.1 um Filtered
• Prepared from Double Distilled and Deionized Water
• Suitable for use in Molecular Applications

This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).

Preparation Note

This product is prepared from double distilled and deionized water

Other Notes

Easily compare specifications for Water products with the Water specification table.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves