MAC506

Mouse Anti-Llama IgG3 Antibody

• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 8E1, monoclonal
• Application: ELISA, WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 8E1, monoclonal
• mol wt: observed mol wt ~47 kDa
• species reactivity: llama
• packaging: antibody small pack of 100 μg
• technique(s): ELISA: suitable, western blot: suitable
• shipped in: dry ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Llama, camels, and alpacas are camelids that produce normal IgG1 like antibodies and also functional IgG isotypes that do not incorporate light chains and possess only heavy chains. These heavy-chain antibodies may constitute about 50% of the IgG in llama serum and as much as 75% of the IgG in camel serum. By virtue of their smaller size they exhibit better bio-distribution, tissue penetration, and monomeric behavior. IgG3 consists of dimers of short heavy chains, which are characterized by a normal Fc region without a CH1 domain. Although it lacks the CH1 region, it still retains an antigen binding domain called the VHH region. Clone 8E1 is shown to specifically bind to IgG3 and not to any of the IgG2 heavy chains. (Ref.: Daley, LP., et al. (2005). Clin. Diag. Lab. Immunol. 12(4); 380-386).

Immunogen

Purified Llama serum IgG3.

Application

Quality Control Testing

Evaluated by Western Blotting with IgG3.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Llama IgG3, but not IgG1 and IgG2.

Tested Applications

Immunoaffinity Purification: A representative lot was used in Immunoaffinity Purification applications (Daley, L.P., et. al. (2005). Clin Diagn Lab Immunol. 12(3):380-6; Daley-Bauer, L.P., et. al. (2010). Clin Vaccine Immunol. 17(12):2007-15).

ELISA Analysis: A representative lot detected Mouse Anti-Llama IgG3 in ELISA applications (Daley, L.P., et. al. (2005). Clin Diagn Lab Immunol. 12(3):380-6; Daley-Bauer, L.P., et. al. (2010). Clin Vaccine Immunol. 17(12):2007-15).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Mouse Anti-Llama IgG3, clone 8E1, Cat. No. MAC506, is a mouse monoclonal antibody that detects Llama IgG3 and is tested for use in ELISA, Immunoaffinity Purification, and Western Blotting.

Biochem/physiol Actions

Clone 8E1 is a mouse monoclonal antibody that detects Llama IgG3.

Physical form

Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable