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UFC201024

Millipore

Amicon® Ultra-2 Centrifugal Filter Unit

Ultracel-10 regenerated cellulose membrane, 2 mL sample volume

Synonym(s):
Centrifugal concentrator, 10 kDa MWCO, 2 mL sample volume
eCl@ss:
36100101
NACRES:
NB.24

Quality Level

material

polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

technique(s)

protein extraction: suitable
protein purification: suitable

L

119.7 mm

diam.

15.9 mm

filtration area

1 cm2

pore size

10 kDa NMWCO

test parameters

sample volume: 2 mL

shipped in

ambient

Application

  • Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates.
  • Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
  • Desalting, buffer exchange and protein dialysis.
  • Size exclusion and protein fractionation.

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
  • Concentrates 2 mL down to 15-70 μL
  • 98% sample recovery
  • Fast processing time: 10 to 30-minute spin times
  • Engineered dead stop
  • Reverse spin capability provides consistent recoveries

Linkage

Replaces: 4205

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Lulai Xu et al.
Vaccine, 38(7), 1690-1699 (2020-01-16)
Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated the thermostable recombinant NDV (rNDV) expressing the different forms of hemagglutinin (HA) of highly pathogenic avian
Bao Quoc Tran et al.
Data in brief, 6, 68-76 (2016-01-23)
A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated
Keerthie Dissanayake et al.
Theriogenology, 149, 104-116 (2020-04-08)
Extracellular vesicles (EVs) are membrane-bound biological nanoparticles (NPs) and have gained wide attention as potential biomarkers. We aimed to isolate and characterize EVs from media conditioned by individually cultured preimplantation bovine embryos and to assess their relationship with embryo quality.
Glenn Vergauwen et al.
Scientific reports, 7(1), 2704-2704 (2017-06-04)
Identification and validation of extracellular vesicle (EV)-associated biomarkers requires robust isolation and characterization protocols. We assessed the impact of some commonly implemented pre-analytical, analytical and post-analytical variables in EV research. Centrifugal filters with different membrane types and pore sizes are
Jasjeet Kaur et al.
Virus genes, 56(4), 480-497 (2020-05-06)
Staphylococcus aureus is one of the most dreadful infectious agents, responsible for high mortality and morbidity in both humans and animals. The increased prevalence of multidrug-resistant (MDR) Staphylococcus aureus strains has limited the number of available treatment options, which calls

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