ENDOLYSS-RO

Endoproteinase Lys-C Sequencing Grade

from Lysobacter enzymogenes

• Synonym(s): lys-c, lysyl endopeptidase, protease
• UNSPSC Code: 12352204
• EC Number: 3.4.21.50 (BRENDA, IUBMB)
• NACRES: NA.54
• eCl@ss: 32160703
• Specific activity: ≥200 units/mg protein (at 25 °C, with Chromozym PL)
• Biological source: bacterial (Lysobacter enzymogenes)

Properties

• biological source: bacterial (Lysobacter enzymogenes)
• form: lyophilized
• specific activity: ≥200 units/mg protein (at 25 °C, with Chromozym PL)
• mol wt: 33 kDa by SDS-PAGE (reducing)
• purified by: electrophoresis
• packaging: pkg of 3 × 5 μg (11047825001), pkg of 5 μg (11420429001)
• manufacturer/tradename: Roche
• storage condition: (Keep container tightly closed in a dry and well-ventilated place.)
• technique(s): activity assay: suitable
• color: white
• optimum pH: 8.5-8.8
• pH range: 5-12
• solubility: water: soluble
• suitability: suitable for enzyme test
• UniProt accession no.: Q7M135
• storage temp.: 2-8°C

Description

General description

Endoproteinase Lys-C Sequencing Grade is isolated from Lysobacter enzymogenes as a highly purified and specific protease. Endoproteinase Lys-C from Lysobacter enzymogenes is a member of the peptidase S1 family. It is mostly used for the initial fragmentation of polypeptide chains in protein sequence analysis.

Application

Use Endoproteinase Lys-C Sequencing Grade  for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.

Biochem/physiol Actions

This enzyme is a serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys (in Tris-HCl buffer, pH 7.0 to 9.0).
Heat inactivation: Enzyme can be denatured using heat (5 min at 100 °C) or by TCA.

Preparation Note

Storage conditions (working solution): +2 to +8 °C

Lyophilized Endoproteinase Lys-C sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 50 mM Hepes, pH 8.0, 10 mM EDTA and 5 mg/ml raffinose. To avoid autolysis, the incubation temperature should not exceed 37 °C.
Stable in 4 M urea.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 11 - Combustible Solids
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash
• Pictograms: GHS08
• Signal Word: Warning
• Hazard Codes: H373
• Precautionary Statements: P260 - P314 - P501
• Hazard Classifications: STOT RE 2
• WGK: WGK 3