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Merck

D8284

Bicinchoninic acid disodium salt hydrate

≥98% purity (HPLC), powder

Synonym(s):

2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt, 4,4′-Dicarboxy-2,2′-biquinoline disodium salt, BCA, Disodium 2,2′-biquinoline-4,4′-dicarboxylate

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1 G

1 480,00 kr

5 G

5 060,00 kr

10 G

9 260,00 kr

25 G

20 120,00 kr

1 480,00 kr

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About This Item

Empirical Formula (Hill Notation):
C20H10N2Na2O4 · xH2O
CAS Number:
979-88-4
Molecular Weight:
388.28 (anhydrous basis)
UNSPSC Code:
12171500
PubChem Substance ID:
24894202
NACRES:
NA.47
MDL number:
MFCD10567219

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Product Name

Bicinchoninic acid disodium salt hydrate, ≥98% (HPLC)

SMILES string

O.[Na+].[Na+].[O-]C(=O)c1cc(nc2ccccc12)-c3cc(C([O-])=O)c4ccccc4n3

InChI

1S/C20H12N2O4.2Na.H2O/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;;/h1-10H,(H,23,24)(H,25,26);;;1H2/q;2*+1;/p-2

InChI key

FAFVGCAYKZKOPT-UHFFFAOYSA-L

assay

≥98% (HPLC)

form

powder

technique(s)

titration: suitable

color

white to light yellow

solubility

0.5% sodium bicarbonate: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Quality Level

200

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1 of 4

This Item
39177894257SML3593
Bicinchoninic acid disodium salt hydrate ≥98% (HPLC)

Sigma-Aldrich

D8284

Bicinchoninic acid disodium salt hydrate

2,2′-Biquinoline-4,4′-dicarboxylic acid dipotassium salt trihydrate 99%

Sigma-Aldrich

391778

2,2′-Biquinoline-4,4′-dicarboxylic acid dipotassium salt trihydrate

Benzophenone-9 analytical standard

Supelco

94257

Benzophenone-9

Hexachlorophene ≥95% (HPLC)

Sigma-Aldrich

SML3593

Hexachlorophene

technique(s)

titration: suitable

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

color

white to light yellow

color

-

color

-

color

white to beige

solubility

0.5% sodium bicarbonate: 50 mg/mL

solubility

-

solubility

-

solubility

DMSO: 2 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

cleaning products
cosmetics
environmental
food and beverages
personal care

application(s)

-

form

powder

form

powder

form

-

form

powder

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

Application

  • Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.[1]
  • It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).[2]
  • It has been used for determining protein concentration.[3]

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.[4]

related product

Product No.
Description
Pricing

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000393754
0000393754
0000492542
0000492542
0000468425

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Prevention of rt-PA induced blood-brain barrier component degradation by the poly(ADP-ribose)polymerase inhibitor PJ34 after ischemic stroke in mice.
Teng F, et al.
Experimental Neurology, 248, 416-428 (2013)
Detection and characterization of chitinases and other chitin-modifying enzymes.
Howard MB, et al.
Journal of Industrial Microbiology & Biotechnology, 30, 627-635 (2003)
Ramya Kumareswaran et al.
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 116(3), 388-391 (2015-04-07)
Solid tumors contain regions of chronic and cyclic hypoxia. Chronic hypoxia can downregulate RAD51 and sensitize cells to PARP inhibition. Herein, we show that RAD51 expression, cell survival and toxicity to PARP inhibition is not affected under cyclic hypoxic conditions.
Rajeev Kumar et al.
Bioresource technology, 130, 372-381 (2013-01-15)
Two established delignification methods employing sodium chlorite-acetic acid (SC/AA) and peracetic acid (PAA) are often used, and are reportedly highly selective. However, these reports are mostly for highly recalcitrant and unpretreated softwoods and hardwoods species, and information for less recalcitrant
Makon-Sébastien Njock et al.
Blood, 125(20), 3202-3212 (2015-04-04)
The blood contains high concentrations of circulating extracellular vesicles (EVs), and their levels and contents are altered in several disease states, including cardiovascular disease. However, the function of circulating EVs, especially the microRNAs (miRNAs) that they contain, are poorly understood.
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