APPA014
HIV Protease Activity Detection Kit
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Synonym(s):
Cut-N-Glow HIV Protease Detection Kit
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Please contact Customer Service for Availability | 11 420,00 kr |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
11 420,00 kr
Save Up To 15 %
Please contact Customer Service for Availability
Application
This kit is specific for the HIV Protease, a retroviral aspartyl protease that is critical to viral life-cycle. The Cut-N-Glow approach involves the introduction of a structural distortion into one of the complementary fragments (GFP 11), through the use of a conditionally stable tether, which serves to constrain the N and C termini of GFP 11 closely in space, thereby, diminishing the mutual affinity of the two fragments and blocking protein self-assembly until the tether is cleaved. The distortion can be reversed upon site-specific proteolysis of the tether, resulting in GFP 11 fragment assembly with GFP 1-10, generating reconstituted, functional GFP. Depending on the tether, the chimeric GFP can serve as a substrate for proteases from the three major classes: serine, cysteine, and aspartic acid.
This kit contains sufficient reagents for one 96 well plate (96 tests)
1 of 1
This Item | |||
|---|---|---|---|
| description - | description 1 kit sufficient for 250 reactions | description sufficient for 20 assays | description N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL |
| shipped in dry ice | shipped in wet ice | shipped in - | shipped in dry ice |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form solution | form - | form - | form aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Storage Class
12 - Non Combustible Liquids
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