The molecular weight for this product has not been determined in-house. Other sources have reported the molecular weight to be 484.7 g/mol.
C0543
Mouse Collagen Type IV
from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation
Synonym(s):
Type IV collagen, EHS-collagen
Skip To
Product Name
Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
biological source
mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
Quality Level
type
Type IV (Miller)
product line
BioReagent
form
lyophilized powder
packaging
glass bottle of 1 vial
technique(s)
cell culture | mammalian: suitable
surface coverage
6‑10 μg/cm2
solubility
0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216)
1 of 4
This Item | C3867 | C7661 | C1188 |
|---|---|---|---|
| biological source mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane) | biological source rat tail | biological source rat tail | biological source bovine cartilage (tracheal) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form liquid | form powder | form powder |
| shipped in dry ice | shipped in wet ice | shipped in - | shipped in - |
| solubility 0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.) | solubility soluble, clear to hazy, colorless (solubilized in 0.02M acetic acid) | solubility soluble | solubility aqueous acid: soluble |
General description
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma.[1] [2]The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.
Application
Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:
Recommended for use as a cell culture substratum at 6-10 μg/cm2.
Biochem/physiol Actions
Preparation Note
0.75 mg/vial
Chloroform treated.
Pepsin is not used in the preparation.
Pepsin is not used in the preparation.
Other Notes
Native collagen
We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Pea3 transcription factor promotes neurite outgrowth
Kandemir B, et al.
Frontiers in Molecular Neuroscience, 7 (2014)
Gels Handbook: Fundamentals, Properties, Applications null
Evaluation of cell binding activities of Leptospira ECM adhesins.
Robbins G T, et al.
PLoS Neglected Tropical Diseases, 9(4), e0003712-e0003712 (2015)
Schwann Cell Autophagy and Necrosis as Mechanisms of Cell Death by Acanthamoeba
Ismael Castelan-Ramirez, et al.
Journal of Separation Science, 9(6), 458-458 (2020)
Mimicking the Extracellular Matrix: The Intersection of Matrix Biology and Biomaterials (2015)
Articles
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
Related Content
-
Could you provide me with the observed molecular weight of the Rat Collagen Type IV product from the Engelbreth-Holm-Swarm murine sarcoma basement membrane? CAS Number:9007-34-5
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service