CORET

PCR Core kit with Taq DNA polymerase

PCR components in separate tubes to allow for optimization

• eCl@ss: 32161000
• NACRES: NA.55

Properties

• Quality Level: 200
• form: liquid
• usage: sufficient for 250 reactions
• feature: dNTPs included; hotstart: no
• technique(s): PCR: suitable
• color: colorless
• input: purified DNA
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. This enzyme is in a recombinant form, expressed in E. coli. Taq DNA polymerase enzyme can withstand high temperatures up to 95 °C without significant loss of activity. Taq DNA Polymerase is a magnesium ion-dependent enzyme. Taq DNA Polymerase possesses 5′→3′ DNA polymerase activity and 5′→3′ exonuclease activity.

Application

CORET PCR kit has been used for the amplification of genomic DNA during the study of genetic diversity in various species of Manilkara zapota.[1]

PCR Core kit with Taq DNA polymerase is suitable for the amplification of genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the polymerase chain reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl₂ will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg²⁺ concentrations and the cycling parameters.

Quantity

Kit contains enough reagents for 250 units of Taq DNA polymerase.

Other Notes

The following reagents are required but not provided in the kit:

• Mineral Oil, M 8662 (optional)
• Thermocycler
• Primers
• DNA to be amplified
• Chloroform, C 7559 (optional)

Legal Information

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit Components Also Available Separately

• P2192 10X PCR Buffer, Optimized for routine PCR with MgCl₂ included 1 mL/vial
• P2317 10X PCR Buffer without MgCl₂, Optimized for routine PCR without MgCl₂ 1 mL/vial
• M8787 Magnesium chloride solution, PCR Reagent, 25 mM MgCI₂ solution for PCR 1 mL/vial
• W1754 Water, PCR Reagent 3 vial(s)
• D7295 Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mL

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable