E7764

Endothelin 1

≥97% (HPLC), powder

• Synonym(s): ET-1
• Empirical Formula (Hill Notation): C₁₀₉H₁₅₉N₂₅O₃₂S₅
• CAS Number: 117399-94-7
• Molecular Weight: 2491.90
• NACRES: NA.77
• PubChem Substance ID: 24894656
• UNSPSC Code: 12352200
• MDL number: MFCD00133305

Properties

• Product Name: Endothelin 1, ≥97% (HPLC), powder
• InChI: 1S/C109H159N25O32S5/c1-12-56(9)87(107(163)125-76(109(165)166)39-60-43-113-65-24-18-17-23-63(60)65)134-108(164)88(57(10)13-2)133-99(155)75(42-85(143)144)123-94(150)70(36-54(5)6)118-97(153)73(40-61-44-112-52-114-61)121-103(159)80-49-169-168-48-64(111)89(145)126-77(45-135)102(158)131-81-50-170-171-51-82(105(161)132-86(55(7)8)106(162)124-72(38-59-26-28-62(138)29-27-59)95(151)120-71(96(152)130-80)37-58-21-15-14-16-22-58)129-91(147)67(30-31-83(139)140)116-90(146)66(25-19-20-33-110)115-98(154)74(41-84(141)142)122-92(148)68(32-34-167-11)117-93(149)69(35-53(3)4)119-100(156)78(46-136)127-101(157)79(47-137)128-104(81)160/h14-18,21-24,26-29,43-44,52-57,64,66-82,86-88,113,135-138H,12-13,19-20,25,30-42,45-51,110-111H2,1-11H3,(H,112,114)(H,115,154)(H,116,146)(H,117,149)(H,118,153)(H,119,156)(H,120,151)(H,121,159)(H,122,148)(H,123,150)(H,124,162)(H,125,163)(H,126,145)(H,127,157)(H,128,160)(H,129,147)(H,130,152)(H,131,158)(H,132,161)(H,133,155)(H,134,164)(H,139,140)(H,141,142)(H,143,144)(H,165,166)/t56-,57-,64-,66-,67-,68-,69-,70-,71-,72-,73-,74-,75-,76-,77-,78-,79-,80-,81-,82-,86-,87-,88-/m0/s1
• SMILES string: [H]N[C@H]1CSSC[C@H](NC(=O)[C@H](Cc2ccccc2)NC(=O)[C@H](Cc3ccc(O)cc3)NC(=O)[C@@H](NC(=O)[C@@H]4CSSC[C@H](NC(=O)[C@H](CO)NC1=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N4)C(C)C)C(=O)N[C@@H](Cc5cnc[nH]5)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc6c[nH]c7ccccc67)C(O)=O
• InChI key: ZUBDGKVDJUIMQQ-ZTNLKOGPSA-N
• biological source: human
• assay: ≥97% (HPLC)
• form: powder
• technique(s): ELISA: suitable
• color: white
• solubility: 1% acetic acid: >1 mg/mL; H₂O: >1 mg/mL
• UniProt accession no.: P05305
• storage temp.: −20°C
• Quality Level: 200
• Gene Information: human ... EDN1(1906)

Description

Application

Endothelin-1 (ET-1) has been used to study the production and detection of H₂O₂ and production of NO in the presence of ET-1,[1] for studying the effects of ET-1 on the eNOS/inducible NOS (iNOS)/caveolin-1 expression, NO production, isoform specific PKC translocation, and PKC activity in human saphenous vein endothelial cells (HSVECs).[2]

Biochem/physiol Actions

Endothelin is a potent agonist for ET_A and ET_B endothelin receptors.

Potent agonist for ET_A and ET_B endothelin receptors. Induces the production of hypoxia-inducible factor 1α, and, thus, the production of VEGF.

Endothelin-1 (ET-1) is a vasoconstrictor, which is increased in plasma and explants of patients with uterine leiomyomas. Thus, uterine fibroids are linked to increased ET-1 levels and hypertension, which is worsened by hypoxic condtions.[3] ET-1 RNAi and Endostar are capable of preventing the progression and invasiveness of PC-3 (prostate cancer) cells.[4] The plasma levels of this peptide are increased in patients with atrial septal defect (ASD), and these levels might help in diagnosis of closure defects.[5] The plasma level of ET-1 is also higher in patients with end-stage renal disease (ESRD), and this is linked to gradual worsening of pulmonary function tests.[6]

General description

Endothelin-1 (ET-1) is synthesized and secreted by activated endothelial cells, and is a peptide with potent vasoconstrictor activity. It is a marker for activated or dysfunctional endothelial cells.[3] This peptide is one of the three isopeptides belonging to the endothelin family. It is also released by cardiomyocytes.[5]

Other Notes

A synthetic peptide with the sequence of human and porcine Endothelin 1.

Lyophilized from 0.1% TFA in H2O

The biological source is human endothelin, which shares the same sequence

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)