On a lot-to-lot basis, no testing is performed during quality control to determine the percentages of the 5 and 6 isomers for this product. No purity testing is conducted to determine the overall dye purity or the purity of the various isomers. The main isomers associated with this product are the 5 and 6 isomers. There is no available information indicating that one isomer is neutrally charged while the other is more negatively charged. According to Conn's Biological Stains, 10th Edition, the 5 and 6 isomers form a lipophilic cation under acidic conditions and a hydrophilic zwitterion around neutrality. It is also noted that the 6-isomer is more strongly fluorescent. If sold in purified form, the 6-isomer is more widely available.
283924
Rhodamine B isothiocyanate
mixed isomers
Rhodamine B isothiocyanate
Synonym(s):
RBITC
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100 MG
$352.00
500 MG
$1,410.00
About This Item
Empirical Formula (Hill Notation):
C29H30ClN3O3S
CAS Number:
Molecular Weight:
536.08
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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Quality Level
form
powder
technique(s)
titration: suitable
color
purple to violet
red to red-brown
solubility
methanol: 10 mg/mL
λmax
555 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1
InChI
1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H
InChI key
YVSWPCCVTYEEHG-UHFFFAOYSA-N
Related Categories
1 of 4
This Item | R8881 | R1755 | R6626 |
|---|---|---|---|
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) - | technique(s) titration: suitable |
| color purple to violet, red to red-brown | color light pink to red, very dark purple | color - | color faint green to very dark green |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing |
| form powder | form powder | form - | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. room temp | storage temp. 2-8°C | storage temp. −20°C | storage temp. room temp |
General description
Rhodamine B isothiocyanate (RBITC) is a fluorescent dye. It acts as an anterograde and retrograde marker of retinal neurons in the adult rat. (current GD)It is also known as RBITC, RhIc, RITC, and TRITC.
Application
Rhodamine B isothiocyanate has been used as a fluorescent probe to label the pathogenic bacterium Staphylococcus aureus and is also used to label type I collagen (C1). Other applications include -
- labeling immunoglobulins
- as a fluorescent probe of living cells
- RBITC-Iabeled molecules like dextrans have been applied as probes of a cell′s endocytic activity and to mark cells to allow their subsequent migration to be detected
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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The use of rhodamine-B-isothiocyanate (RITC) as an anterograde and retrograde tracer in the adult rat visual system.
Thanos S
Brain Research, 406, 317-321 (1987)
Labeling of the pathogenic bacterium Staphylococcus aureus with gold or ferric oxide-core nanoparticles highlights new capabilities for investigation of host?pathogen interactions
Depke M
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 85, 140-150 (2014)
Transdermal Delivery of Functional Collagen Via Polyvinylpyrrolidone Microneedles
Sun W
Annals of Biomedical Engineering, 43, 2978-2990 (2015)
Modification of fluorescent-antibody procedures employing crystalline tetramethylrhodamine isothiocyanate.
M L SMITH et al.
Journal of bacteriology, 83, 1358-1359 (1962-06-01)
Drug dissolution chip (DDC): a microfluidic approach for drug release.
Maike Windbergs et al.
Small (Weinheim an der Bergstrasse, Germany), 7(21), 3011-3015 (2011-09-16)
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This mixture is composed specifically what isomers? when characterized with capillary electrophoresis, getting 2 peaks, one being neutrally charged and another one being more negatively charged. Which one is used to label immunoglobulins?
1 answer-
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