No, bacteria are below the size threshold for counting on the Scepter. Sensor tips are designed to be single use.
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description
Rapid cell counts in a handheld easy to use format, includes pkg of 40 μm Scepter 3.0 Cell Counter Sensors
manufacturer/tradename
Scepter
technique(s)
cell counting: suitable
particle size
4-18 μm
compatibility
for use with cells sized 4.0–25.0 μm
shipped in
ambient
General description
- Cell concentration
- Average cell size
- Average cell volume
- Histogram of size or volume distribution
The Scepter 3.0 Cell Counter is compatible with both the 40uM and 60uM Scepter 3.0 Cell Counter Sensors. This kit (PHCC340KIT) includes a free 50-pack of 40uM sensors.
Other Notes
- 1 Scepter 3.0 Automated Handheld Cell Counter
- 1 Test Bead Vial
- 1 Charging Station
- 50 Sensors (40μM)
accessory
Storage Class Code
10-13 - German Storage Class 10 to 13
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.
Cell counting protocol using a hemocytometer for monitoring cell viability. Discover other cell counting tools such as the Scepter™ 3.0 Cell Counter.
Find answers to frequently asked questions about the Millicell® ERS 3.0, from probe depth adjustment to instrument maintenance and troubleshooting.
The Scepter™ 3.0 Cell Counter, when used in conjunction with a 40 μm aperture sensor, can accurately count and precisely size a wide range of cell types and particles. This application note demonstrates two examples of experiments using the instrument’s sensitive size-discriminating capacity for rapid qualitative assessment of individual cell populations.
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Scepter™ counting is 7 to 10 times faster than hemocytometry-and also faster than other automated counters. Learn more about how this amazing technology works
A list of which mammalian and other key phenotypes have been validated for Scepter™ counting precision.
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Will Scepter 3.0 be able to quantify bacterial cultures? How many reads is it possible with a single counter sensor?
1 answer-
Helpful?
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Can it count nuclei, specifically isolated from human aortic endothelial cells?
1 answer-
While most mammalian nuclei are around 6 micrometers (um), which is at the low end of detection, this size range can also include other cell debris that is often gated out during analysis. Additionally, since the counts are displayed as a normal distribution, the full profile may not be visible at the low end of detection. Generally, it's not recommended to use this method for small nuclei, but the Scepter may work for some cells with larger nuclei.
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