The thickness of the PTFE membrane for the PICM01250 inserts is approximately 65 micrometers (um), and these inserts are compatible with inverted microscopes.
PICM01250
Millicell® 24 well standing inserts
pore size 0.4 μm, diam. 12 mm, transparent PTFE membrane, hydrophilic, sterile
Synonym(s):
Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE, 0.4 µm, Millicell-CM, cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate
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About This Item
UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22
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material
polystyrene housing
transparent PTFE membrane
Quality Level
sterility
ethylene oxide treated
sterile
feature
hydrophilic
packaging
pack of 50 (individually blister packaged)
manufacturer/tradename
Millicell®
parameter
50 °C max. temp.
technique(s)
cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
H
10.5 mm
diam.
12 mm
filtration area
0.6 cm2
size
24 wells
surface area
0.6 cm2
working volume
0.4 mL
color
transparent membrane, when wetted
matrix
Biopore™
pore size
0.4 μm
binding type
low binding surface
detection method
fluorometric
shipped in
ambient
Related Categories
General description
With Millicell® standing inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® standing inserts make it possible to study both sides of the cell monolayer.
Application
- Cell Attachment
- Cell Growth
- Cell Differentiation
- Immunocytochemistry
Features and Benefits
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies
- Low protein-binding hydrophilic PTFE membrane is ideal for live cell viewing and immunofluorescent application
Legal Information
BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
accessory
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Description
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Description
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
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Tong-Dian Zhang et al.
Oxidative medicine and cellular longevity, 2020, 4569268-4569268 (2020-06-23)
Mono-(2-ethylhexyl) phthalate (MEHP) and genistein have been classified as endocrine-disrupting chemicals (EDCs) which interfere with the differentiation and development of the male reproductive system. However, how these two EDCs would affect fetal rat testis development at a low dose was
Ming Zeng et al.
Gene, 753, 144810-144810 (2020-05-30)
Meiotic entry and progression require dynamic regulation of germline gene expression. m6A on mRNAs and recognition by YTHDC2 has been known as post-transcriptional regulatory complex, but the roles of this regulator remain unclear for meiotic initiation and progression in female
Gravity in mammalian organ development: differentiation of cultured lung and pancreas rudiments during spaceflight.
Spooner, B S, et al.
The Journal of Experimental Zoology, 269, 212-222 (1994)
Weijie Yang et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(4), 1200-1213 (2020-02-26)
In mammals, resting primordial follicles serve as the ovarian reserve. The decline in ovarian function with aging is characterized by a gradual decrease in both the quantity and quality of the oocytes residing within the primordial follicles. Many reports show
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane
Articles
16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.
Protocols
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This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.
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Related Content
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
Streamline TEER data capture and analysis with user-friendly enhancements including an intuitive touchscreen interface, standing in-well probe, and automatic data logging.
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