LYSISO1
Lysosome Isolation Kit
sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells
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LYSISO1-1KT
$899.00
$899.00
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About This Item
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Quality Level
usage
sufficient for 20 mL (packed cells)
sufficient for 25 g (tissue)
technique(s)
centrifugation: suitable
fractionation: suitable
storage temp.
2-8°C
1 of 4
This Item | PEROX1 | MAK172 | MAK456 |
|---|---|---|---|
| technique(s) centrifugation: suitable, fractionation: suitable | technique(s) centrifugation: suitable, fractionation: suitable | technique(s) - | technique(s) - |
| usage sufficient for 20 mL (packed cells) | usage - | usage sufficient for 100 fluorometric tests | usage - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 100 |
General description
Lysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells. They contain many acid hydrolases that take part in protein degradation in the cell. Lysosomes also contain lipases, nucleases and polysaccharidases and deficiencies in some of these enzymes lead to specific lysosomal storage diseases such as Tay Sachs, Gaucher, Hunter disease and others. They also contribute to maintaining cellular homeostasis. Malfunctions in this organelle directly impact cell behavior and fate. Lysosomes may also be involved in other cellular processes such as Albinism and aging.
Application
Lysosome Isolation Kit has been used:
The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.
- in the isolation of cytosol and lysosome[1]
- to obtain enriched light and heavy lysosomal fractions from cultured cells[2]
- in the isolation of lysosomes of mouse bone marrow macrophages (BMMs)[3]
- in lysosome purification[4]
The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.
Features and Benefits
- Generates functional organelles for metabolism and disease research
- Includes protease inhibitor to manage degradation
- Measures integrity with included Neutral Red dye
- Choose from multiple options to obtain enriched fractions
- Enriched fractions for intact and functional lysosomes from tissues and cells
- Suitable for functional studies including protein degradation
Kit Components Also Available Separately
Product No.
Description
SDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mL
used together
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
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Li X, et al.
PLoS ONE, 9(1), e87552-e87552 (2014)
Glycine N-methyltransferase deficiency affects Niemann-Pick type C2 protein stability and regulates hepatic cholesterol homeostasis
Liao YJ, et al.
Molecular Medicine, 18(3), 412-422 (2012)
Quantitative proteomic profiling for clarification of the crucial roles of lysosomes in microbial infections
Xu B, et al.
Molecular Immunology, 87(1), 122-131 (2017)
Fang-Shin Nian et al.
Molecular neurobiology, 56(9), 6095-6105 (2019-02-06)
Mutations in RAB18, a member of small G protein, cause Warburg micro syndrome (WARBM), whose clinical features include vision impairment, postnatal microcephaly, and lower limb spasticity. Previously, our Rab18-/- mice exhibited hind limb weakness and spasticity as well as signs
Articles
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
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