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Merck

MSIPS4W10

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, sterile

Synonym(s):

Hydrophobic 96-Well Plate, PVDF 96-Well Plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32039006
NACRES:
NB.22

Key Documents

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material

Durapore PVDF membrane
flat bottom wells
white acrylic plate

Quality Level

400

description

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

sterility

sterile

usage

Automated

feature

hydrophobic
lid

packaging

pkg of 10 plates

manufacturer/tradename

MultiScreen®

technique(s)

ELISPOT: suitable
cell based assay: suitable
dot blot: suitable

filtration area

0.28 cm2

plate size

96 wells

well maximum volume

300 μL

working volume

50-250 μL

matrix

Immobilon®-P

pore size

0.45 μm pore size

binding type

high binding surface

compatibility

centrifuge & manifold

shipped in

ambient

Related Categories

General description

The polyvinylidene fluoride (PVDF) membrane plates are recommended, over a mixed cellulose ester format, due to slightly improved binding of capture antibody and superior performance in spot detection, particularly for fluorescent applications.

Application

MultiScreen® HTS IP Filter Plate, 0.45 μm, white, sterile has been used in enzyme-linked immunospot (ELISpot) assay using:
  • dsDNA-producing plasma cells
  • memory B cells and bone marrow localized long-lived plasma cells
  • draining lymph node and spleen cells

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Katrin Klocke et al.
Immunology, 152(1), 125-137 (2017-05-13)
Cytotoxic T-lymphocyte antigen 4 (CTLA-4) -mediated regulation of already tolerized autoreactive T cells is critical for understanding autoimmune responses. Although defects in CTLA-4 contribute to abnormal FOXP3+ regulatory T (Treg) cell function in rheumatoid arthritis, its role in autoreactive T
Mubeen M Mosaheb et al.
Nature communications, 11(1), 524-524 (2020-01-29)
Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of
Leah E Cole et al.
Vaccines, 8(4) (2020-12-06)
While both whole-cell (wP) and acellular pertussis (aP) vaccines have been highly effective at reducing the global pertussis disease burden, there are concerns that compared to wP vaccination, the immune responses to aP vaccination may wane more rapidly. To gain
Jing Zhu et al.
Journal of immunology (Baltimore, Md. : 1950), 204(5), 1091-1100 (2020-01-29)
Almost a decade has passed since the approval of belimumab, an mAb directed against B lymphocyte stimulation and the first targeted therapy approved for systemic lupus erythematous (SLE) in over 50 y. Although well tolerated, the efficacy of belimumab remains

Articles

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

Protocols

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

IFN-γ ELISpot Assays on MultiScreen® IP

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Related Content

Chemical Compatibility Guide for MultiScreen® Filter Plates

This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.

MultiScreen®HTS and MultiScreen®HTS+ Hi Flow Assay Systems
MultiScreen®Filter Plates Poster

MultiScreen® Filter Plates

Elispot assays: state-of-the-art tools for functional analysis of cellular immunology White Paper

"Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information. By virtue of this assay’s unsurpassed sensitivity, frequency analysis of rare cell populations (e.g., antigen-specific responses) which were not possible before are now relatively easy. Recent improvements to the design of multiwell microplates, including use of membranes with reduced background fluorescence, have bolstered the widespread application of Elispot assays. When assay sensitivity, ease of use, and cost are all taken into consideration, the Elispot platform is likely the superior choice for the development of multifunctional T cell assays for the research, therapeutic, and diagnostic communities."

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