AB10526

Anti-COX4 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): cytochrome c oxidase subunit IV isoform 1, cytochrome c oxidase subunit IV1, Cytochrome c oxidase polypeptide IV, cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NP_001852
• UniProt accession no.: P13073
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... COX4I1(1327)

Description

General description

Cytochrome C oxidase subunit 4 (COX4), is one of 13 subunits of cytochrome c oxidase. Cytochrome c oxidase is responsible for catalyzing the last step of electron transfer through the respiratory chain on the mitochondrial inner membrane. COX4 assembles into sub-complexes with other subunits of cytochrome c oxidase and may represent a rate-limiting intermediate.

~17 kDa observed.
A non-specific band may be observed at ~58 kDa

Immunogen

KLH-conjugated linear peptide corresponding to human COX4.

Application

Anti-COX4 Antibody detects level of COX4 & has been published & validated for use in WB, IH(P).

Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected COX4 in normal human cardiac muscle tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

Other homologies: Rat and mouse (59% sequence homology).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human fetal skeletal muscle tissue lysate

Evaluated by Western Blot in human fetal skeletal muscle tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected COX4 in 10 µg of human fetal skeletal muscle tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.