As provided, this antibody does not have a fluorescent molecule conjugated to it and would need a secondary antibody labeled with a fluorophore for visualization by fluorescent signal.
CBL186
Anti-DNA Antibody, clone AC-30-10
clone AC-30-10, Chemicon®, from mouse
Synonym(s):
Deoxyribonucleic acid
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AC-30-10, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
isotype
IgM
shipped in
wet ice
target post-translational modification
unmodified
Application
Anti-DNA Antibody, clone AC-30-10 is a Mouse Monoclonal Antibody for the detection of DNA & has been validated in IF, IHC(P).
Research (cell biology, molecular biology, microbiology) since it reacts with any form of native and denatured DNA. Reliable positive control in immunofluoresence assays for detecting DNA antibodies on tissues of cultured cells, notably in the diagnosis of autoantibodies and autoimmune diseases.
Sensitive detection of mycoplasma contamination in cell cultures.
For frozen and paraffin tissue sections.
Optimal working dilutions must be determined by the end user.
Sensitive detection of mycoplasma contamination in cell cultures.
For frozen and paraffin tissue sections.
Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Biochem/physiol Actions
This antibody reacts with native denatured DNA extracted from various organisms: mouse DNA, calf thymus DNA, M13 DNA, SV-40 DNA and several plasmids. It also reacts with synthetic DNA of different base compositions: poly dAT-poly dAT, poly dAT. DNA-containing structures such as chromatin in diverse tissues from several species as well as in diverse cell lines such as XLKE-A6 (Xenopus), RVF-SMC (rat kangaroo) and HeLa (human) are also recognized.
Physical form
Format: Purified
The immunoglobulin was purified by gel filtration and presented in PBS, pH 7.4, containing 0.09% sodium azide and 0.5% BSA.
Preparation Note
Maintain at 2-8°C for up to 12 months from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jessica W Luzwick et al.
Science advances, 7(51), eabf9441-eabf9441 (2021-12-16)
Mitochondrial DNA (mtDNA) instability activates cGAS-dependent innate immune signaling by unknown mechanisms. Here, we find that Fanconi anemia suppressor genes are acting in the mitochondria to protect mtDNA replication forks from instability. Specifically, Fanconi anemia patient cells show a loss
Ya-Jing Li et al.
Acta pharmacologica Sinica, 43(8), 2026-2041 (2022-01-15)
Liver fibrosis is the common consequence of almost all liver diseases and has become an urgent clinical problem without efficient therapies. Recent evidence has shown that hepatocytes-derived extracellular vesicles (EVs) play important roles in liver pathophysiology, but little is known
Andong Qin et al.
Cells, 12(21) (2023-11-10)
Coxsackievirus B3 (CVB3), a single-stranded positive RNA virus, primarily infects cardiac myocytes and is a major causative pathogen for viral myocarditis (VMC), driving cardiac inflammation and organ dysfunction. However, whether and how myocardial damage is involved in CVB3-induced VMC remains
Mohammad T Hussan et al.
Current issues in molecular biology, 44(3), 1215-1223 (2022-06-21)
The presence of ectopic DNA in the cytoplasm induces inflammation and cell death. It has been widely reported that leakage of nuclear DNA into the cytoplasm can mainly be sensed by cyclic GMP-AMP synthase (cGAS). We recently reported that mitochondria-derived
Pedro Silva-Pinheiro et al.
Nucleic acids research, 49(9), 5230-5248 (2021-05-07)
Mutations in POLG, encoding POLγA, the catalytic subunit of the mitochondrial DNA polymerase, cause a spectrum of disorders characterized by mtDNA instability. However, the molecular pathogenesis of POLG-related diseases is poorly understood and efficient treatments are missing. Here, we generate
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Hi, I am wondering if this Antibody has the fluorophore itself already or a secondary conjugated antibody is needed for fluorescence? Thank you, Liye
1 answer-
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