Amicon Ultra-2 devices should be used in a centrifuge with swinging bucket or fixed angle rotor with wells/carriers that can accommodate 17 mm × 100 mm tubes. This is the same well/carrier size that is recommended for the Amicon® Ultra-4 devices. To avoid damage to the device during centrifugation, make sure it is properly assembled and seated at the bottom of the rotor. The rim of the concentrate collection tube should be inside the rotor well. Check clearance before spinning.
UFC201024
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa
Synonym(s):
Centrifugal concentrator, 10 kDa MWCO, 2 mL sample volume
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About This Item
UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
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material
polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
2 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
119.7 mm
diam.
15.9 mm
filtration area
1 cm2
volume
2 mL
working volume
≤2 mL
2 mL
pore size
10 kDa MWCO
greener alternative category
shipped in
ambient
General description
Amicon® Ultra-2 centrifugal filter devices offer fast ultrafiltration. It has the potential to recover high concentration factors and easy concentrate from dilute and complex sample matrices. Amicon® Ultra-2 devices are supplied non-sterile and are for single use only.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates.
- Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Amicon® Ultra-2 Centrifugal Filter Unit has been used:
- to reduce large volume biofluids prior to extracellular vesicle (EV) isolation or to concentrate EVs[1]
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids (DNA/RNA samples, either single- or double-stranded), microorganisms, column eluates, and purified samples
- Purification of macromolecular components found in tissue culture extracts and cell lysates, removal of primer, linkers, or molecular labels from a reaction mix, and protein removal before high-performance liquid chromatography (HPLC)
- Desalting, buffer exchange[2], or diafiltration
Features and Benefits
- Amicon® Ultra 2ml 10K 24PK
- Concentrates 2 mL down to 15-70 μl
- 98% sample recovery
- Fast processing time - 10 to 30 minute spin times
- Engineered dead stop
- The reverse spin capability provides consistent recoveries
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 2 mL down to 15-70 μL
- 98% sample recovery
- Fast processing time: 10 to 30-minute spin times
- Engineered dead stop
- Reverse spin capability provides consistent recoveries
Other Notes
Replaces: 4205
The Amicon® Ultra-2 device is supplied with two tubes. During operation, one tube is used to collect filtrate; the other to cap the device during concentration and subsequently to recover the concentrated sample. Amicon Ultra-2 Centrifugal filter devices are for research use only and not for use in diagnostic procedures.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lulai Xu et al.
Vaccine, 38(7), 1690-1699 (2020-01-16)
Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated the thermostable recombinant NDV (rNDV) expressing the different forms of hemagglutinin (HA) of highly pathogenic avian
Bao Quoc Tran et al.
Data in brief, 6, 68-76 (2016-01-23)
A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated
Keerthie Dissanayake et al.
Theriogenology, 149, 104-116 (2020-04-08)
Extracellular vesicles (EVs) are membrane-bound biological nanoparticles (NPs) and have gained wide attention as potential biomarkers. We aimed to isolate and characterize EVs from media conditioned by individually cultured preimplantation bovine embryos and to assess their relationship with embryo quality.
Glenn Vergauwen et al.
Scientific reports, 7(1), 2704-2704 (2017-06-04)
Identification and validation of extracellular vesicle (EV)-associated biomarkers requires robust isolation and characterization protocols. We assessed the impact of some commonly implemented pre-analytical, analytical and post-analytical variables in EV research. Centrifugal filters with different membrane types and pore sizes are
Jasjeet Kaur et al.
Virus genes, 56(4), 480-497 (2020-05-06)
Staphylococcus aureus is one of the most dreadful infectious agents, responsible for high mortality and morbidity in both humans and animals. The increased prevalence of multidrug-resistant (MDR) Staphylococcus aureus strains has limited the number of available treatment options, which calls
Articles
สำรวจหลักการของเมมเบรนกรองและประโยชน์สำหรับการเตรียมตัวอย่างโปรตีนรวมถึงการแลกเปลี่ยนตัวทำละลายและความเข้มข้นของตัวอย่าง
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Protocols
แนวทางปฏิบัติที่ดีที่สุดสำหรับการสร้างมาตรฐานและลดขั้นตอนการเตรียมถุงลมนอกเซลล์และขจัดการปนเปื้อนของการเตรียม EV ด้วยถุงลมที่ได้จากเซลล์ตายแล้ว
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
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"The Amicon® Ultra 2 mL filter provides a fast and easy way to concentrate, desalt and exchange thebuffer for both proteins and nucleic acids. Now you can concentrate 2 mL down to 15 – 70 μL in anAmicon® Ultra centrifugal device."
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Can Amicon® Ultra Centrifugal Filter, 10 kDa MWCO UFC201024 to be in the Centrifuge as 4 mL Centrifugal Filter without adaptors？We have adaptors for 15 mL TUBES
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Hello. Does these 2 ml 10 kDa filters fit into the same centrifuge rotor as the 0.5 ml 10 kDa amicon filters?
1 answer-
No, the Amicon-0.5 devices require a microfuge, while the Amicon-2 devices require a centrifuge with adapters for 5 ml tubes.
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