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556416

New Methylene Blue N

Dye content ~70 %

Synonym(s):

Basic Blue 24, Methylene Blue N

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About This Item

Empirical Formula (Hill Notation):
C18H22ClN3S
CAS Number:
1934-16-3
Molecular Weight:
347.91
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24879556
EC Number:
217-696-9
Colour Index Number:
52030
MDL number:
MFCD00135878

Key Documents

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Product Name

New Methylene Blue N, Dye content ~70 %

InChI key

NZYCYASKVWSANA-UHFFFAOYSA-M

InChI

1S/C18H22N3S.ClH/c1-5-19-13-9-17-15(7-11(13)3)21-16-8-12(4)14(20-6-2)10-18(16)22-17;/h7-10,19-20H,5-6H2,1-4H3;1H/q+1;/p-1

SMILES string

[Cl-].CCNc1cc2[s+]c3cc(NCC)c(C)cc3nc2cc1C

form

powder, crystals or chunks

composition

Dye content, ~70%

technique(s)

microbe id | staining: suitable

mp

190 °C (dec.) (lit.)

λmax

590 nm
632 nm (2nd)

εmax

≥29000 at 626-634 nm in water
≥30500 at 586-594 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Show Differences

1 of 4

This Item
202096M5528M9140
New Methylene Blue N Dye content ~70 %

Sigma-Aldrich

556416

New Methylene Blue N

New Methylene Blue N zinc chloride double salt Dye content 90 %

Sigma-Aldrich

202096

New Methylene Blue N zinc chloride double salt

Methyl Blue certified by the BSC

Sigma-Aldrich

M5528

Methyl Blue

Methylene blue certified by the BSC

Sigma-Aldrich

M9140

Methylene blue

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder, crystals or chunks

form

powder

form

powder

form

powder

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

composition

Dye content, ~70%

composition

Dye content, 90%

composition

-

composition

Dye content, ≥82%

mp

190 °C (dec.) (lit.)

mp

>240 °C (dec.) (lit.)

mp

-

mp

-

Application

New methylene blue N has been used as a photosensitizer for the photodynamic inactivation of Candida albicans.[1][2]

Biochem/physiol Actions

New methylene blue N can bind, neutralize and crosslink with RNA. This causes co-precipitation of ribosomal and residual RNA with the mitochondria and ferritin masses. These events make the living young erythrocytes as microscopically visible, dark-blue clusters and filaments, referred to as reticulum. New Methylene Blue N helps in counting of reticulocytes (erythrocytes containing RNA).[3]

Features and Benefits

Solvent soluble cationic thiazine dye

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000376807
0000376807
0000164445
0000164445
SHBN9708

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Immature reticulocyte fraction as a criterion for marrow engraftment. Evaluation of a semi-automated reticulocyte counting method.
H Z Grotto et al.
Clinical and laboratory haematology, 21(4), 285-287 (1999-12-03)
J O'Neill et al.
Journal of chemotherapy (Florence, Italy), 15(4), 329-334 (2003-09-10)
In order to establish a comparative order of efficacy among established photosensitising compounds currently under investigation, the in vitro photobactericidal activities of six commercially available photosensitisers were investigated at equal concentration against Streptococcus sanguis using a Helium Neon (HeNe) laser
I Rabaey et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 52(1-2), 515-523 (2005-09-27)
Microbial fuel cells which are operated in continuous mode are more suitable for practical applications than fed batch ones. Three influent types containing carbohydrates were tested, i.e. a glucose medium, a plant extract and artificial wastewater. The anode reactor compartment
Tianhong Dai et al.
Antimicrobial agents and chemotherapy, 55(12), 5710-5717 (2011-09-21)
The objective of this study was to investigate photodynamic therapy (PDT), using blue dye and red light, for prophylaxis and treatment of cutaneous Candida albicans infections in mice. A mouse model of skin abrasion infected with C. albicans was developed
S K Lai et al.
Clinical and laboratory haematology, 21(4), 261-264 (1999-12-03)
Hb-H disease is a form of alpha-thalassemia commonly found in south-east Asia. In this condition, numerous Hb-H bodies are found in erythrocytes using reticulocyte staining preparations. This is the first study addressing the possible interference of Hb-H inclusions in automated
View All Related Papers

Questions

  1. I would like to use this to stain blood smears to identify/count reticulocytes. What volume of stain would 1g of the powder make?

    1 answer

    1. Instructions for use are not available for this product. However, product R4132 is a prepared solution specifically for use in reticulocyte staining. The New Methylene Blue concentration of this solution is 1% or 1g/100 mL The product formulation is as follows:
      New Methylene blue, 1%
      Sodium Chloride, 0.9%
      Potassium Oxalate, 1.6%

      Based on the formulation listed above, the end-user will be able to prepare 100 ml of a 1% solution if using product 556416. This would also require the addition of the sodium chloride and potassium oxalate components.
      Please see the link below to review the product datasheet for the prepared stain R4132:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/236/942/procedure-r4132-reticulocytestain-ms.pdf

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