D8284

Bicinchoninic acid disodium salt hydrate

≥98% purity (HPLC), powder

• Synonym(s): 2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt, 4,4′-Dicarboxy-2,2′-biquinoline disodium salt, BCA, Disodium 2,2′-biquinoline-4,4′-dicarboxylate
• Empirical Formula (Hill Notation): C₂₀H₁₀N₂Na₂O₄ · xH₂O
• CAS Number: 979-88-4
• Molecular Weight: 388.28 (anhydrous basis)
• MDL number: MFCD10567219
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894202
• NACRES: NA.47

Properties

• Product Name: Bicinchoninic acid disodium salt hydrate, ≥98% (HPLC)
• Quality Level: 200
• Assay: ≥98% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: white to light yellow
• solubility: 0.5% sodium bicarbonate: 50 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: O.[Na+].[Na+].[O-]C(=O)c1cc(nc2ccccc12)-c3cc(C([O-])=O)c4ccccc4n3
• InChI: 1S/C20H12N2O4.2Na.H2O/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;;/h1-10H,(H,23,24)(H,25,26);;;1H2/q;2*+1;/p-2
• InChI key: FAFVGCAYKZKOPT-UHFFFAOYSA-L

Description

Application

• Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.[1]
• It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).[2]
• It has been used for determining protein concentration.[3]

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu⁺), in presence of alkaline conditions. The amount of Cu⁺ is in relation with the protein concentration.[4]

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves