G2267

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle

Name: Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle
Brand: SIGMA

lyophilized powder, ≥75 units/mg protein

Synonym(s):

D-Glyceraldehyde 3-phosphate:NAD⁺ oxidoreductase (phosphorylating), GAPDH

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About This Item

CAS Number:

9001-50-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-609-4

MDL number:

MFCD00131203

EC Number:

1.2.1.12 (BRENDA, IUBMB)

Key Documents

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Product Name

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle, lyophilized powder, ≥75 units/mg protein

biological source

rabbit muscle

form

lyophilized powder

specific activity

≥75 units/mg protein

composition

Protein, ≥80% biuret

foreign activity

3-Phosphoglyceric phosphokinase ≤0.05%
lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%
triosephosphate isomerase ≤0.1%

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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Chemistry & Biochemicals

Phosphates

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Show Differences

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This Item	G6880	P3397	P6635
Sigma-Aldrich G2267 Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle	Sigma-Aldrich G6880 α-Glycerophosphate Dehydrogenase from rabbit muscle	Sigma-Aldrich P3397 Phosphoglucomutase from rabbit muscle	Sigma-Aldrich P6635 Phosphorylase b from rabbit muscle
biological source rabbit muscle	biological source -	biological source rabbit muscle	biological source rabbit muscle
specific activity ≥75 units/mg protein	specific activity ≥100 units/mg protein	specific activity ≥100 units/mg protein	specific activity ≥20 units/mg protein
form lyophilized powder	form lyophilized powder	form ammonium sulfate suspension	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in -
foreign activity 3-Phosphoglyceric phosphokinase ≤0.05%, triosephosphate isomerase ≤0.1%, lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%	foreign activity Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, Triosephosphate isomerase ≤0.02%	foreign activity lactic dehydrogenase ≤0.5%, phosphoglucose isomerase ≤0.01%, pyruvate kinase ≤0.05%	foreign activity phosphoglucomutase ≤1.0%, phosphorylase a ≤10%, phosphorylase kinase ≤0.5%, phosphorylase phosphatase, debrancher enzyme, AMPase and ATPase ≤0.1%

Application

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:

for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay[1]
to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice[2]
in anti-aggregation assays[3]
in purified GAPDH studies[4]

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.

General description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein.[5] It is basically called as a glycolytic enzyme.[6]

Other Notes

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing citrate buffer salts

related product

Product No.

Description

Pricing

I2512

Sodium iodoacetate, ≥98%

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Oligomerization of a molecular chaperone modulates its activity

Saio T, et al.

eLife, 7(6), e35731-e35731 (2018)

Poly ADP-Ribose Polymerase Inhibition Ameliorates Hind Limb Ischemia Reperfusion Injury in a Murine Model of Type 2 Diabetes

Long C A, et al.

Annals of Surgery, 258(6) (2013)

Role of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in DNA repair

Kosova A A, et al.

The Journal of Biological Chemistry, 82(6), 643-654 (2017)

Paradoxical inhibition of glycolysis by pioglitazone opposes the mitochondriopathy caused by AIF deficiency

Benit P, et al.

EBioMedicine, 17, 75-87 (2017)

The Phosphoglycerate Kinase (PGK) Gene Family of Maize (Zea mays var. B73).

Julio A Massange-Sánchez et al.

Plants (Basel, Switzerland), 9(12) (2020-12-02)

Phosphoglycerate kinase (PGK, E.C. 2.7.2.3) interconverts ADP + 1,3-bisphospho-glycerate (1,3-bPGA) to ATP + 3-phosphoglycerate (3PGA). While most bacteria have a single pgk gene and mammals possess two copies, plant genomes contain three or more PGK genes. In this study, we

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