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S2563

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp.

Synonym(s):

SCDase

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About This Item

UNSPSC Code:
12352211
NACRES:
NA.32
MDL number:
MFCD02100603

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foreign activity

exoglycosidases: α- and β-galactosidase, α- and β-N-acetylgalactosaminidase, β-N-acetylglucosaminidase, α-mannosidase, α-fucosidase, and sialidase., essentially free
protease and sphingomyelinase, essentially free

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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This Item
G7509A3352C1403
Sphingolipid Ceramide N-Deacylase from Pseudomonas sp.

Sigma-Aldrich

S2563

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp.

GABase from Pseudomonas fluorescens lyophilized powder, Protein ≥30 % by biuret

Sigma-Aldrich

G7509

GABase from Pseudomonas fluorescens

Acyl-coenzyme A Synthetase from Pseudomonas sp. ≥2 units/mg protein

Sigma-Aldrich

A3352

Acyl-coenzyme A Synthetase from Pseudomonas sp.

Cholesterol Esterase from Pseudomonas sp. lyophilized powder, ≥200,000 units/g protein

Sigma-Aldrich

C1403

Cholesterol Esterase from Pseudomonas sp.

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

-

foreign activity

exoglycosidases: α- and β-galactosidase, α- and β-N-acetylgalactosaminidase, β-N-acetylglucosaminidase, α-mannosidase, α-fucosidase, and sialidase., essentially free

foreign activity

-

foreign activity

-

foreign activity

-

Application

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. has been used in enzymatic conversion and derivatization of sulfatides.[1] It has also been used to hydrolyze the fatty acid chain of mactosyl ceramide.[2]

Biochem/physiol Actions

Hydrolyzes the N-acyl linkage between fatty acids and sphingosines in ceramides of various sphingolipids.
Sphingolipid Ceramide N-Deacylase from Pseudomonas sp., under certain conditions, can recyclate lyso-sphingolipids.[3] It condenses fatty acids to sphingosine to generate ceramide.[4] The enzyme mainly acts on neutral and acidic glycosphingolipids.[5]

General description

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. is a hydrolytic enzyme.[3]

Other Notes

One unit will hydrolyze 1 μmol of asialo GM1 per minute at pH 6.0 at 37 °C.

Physical form

Solution in 50 mM sodium acetate, pH 6.0, with 0.1% Lubrol PX.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000350902
0000350892
0000350902
0000350892
SLCM7088

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Sulfatide Analysis by Mass Spectrometry for Screening of Metachromatic Leukodystrophy in Dried Blood and Urine Samples.
Spacil Z
Clinical Chemistry, 62(1), 279-286 (2016)
Characterization of the reversible nature of the reaction catalyzed by sphingolipid ceramide N-deacylase. A novel form of reverse hydrolysis reaction.
Kita K
European Journal of Biochemistry, 268(3), 592-602 (2001)
L Kuchar et al.
Rapid communications in mass spectrometry : RCM, 24(16), 2393-2399 (2010-07-17)
Sphingolipid ceramide N-deacylase (SCDase, EC 3.5.1.69) is a hydrolytic enzyme isolated from Pseudomonas sp. TK 4. In addition to its primary deacylation function, this enzyme is able to reacylate lyso-sphingolipids under specific conditions. We immobilised this enzyme on magnetic macroporous
D Thusitha U Abeytunga et al.
Bioorganic chemistry, 36(2), 70-76 (2007-11-21)
Glycosphingolipids (GSLs) have been implicated as playing major roles in cellular interactions and control of cell proliferation in muticellular organisms. Moreover GSLs and other sphingolipids such as sphingomyelins, ceramides and sphingosines serve a variety of roles in signal transduction. Hence
A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycosphingolipids as well as sphingomyelin to produce their lyso forms.
Ito M
The Journal of Biological Chemistry, 270(41), 24370-24374 (1995)

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