A9528

Amphotericin B solubilized

powder, suitable for cell culture, BioXtra

• Synonym(s): Amphotericin B from Streptomyces sp.
• สูตรเอมพิริกัล (Hill Notation): C₄₇H₇₃NO₁₇
• CASE番号: 1397-89-3
• น้ำหนักโมเลกุล: 924.08
• UNSPSC Code: 12352207
• eCl@ss: 34010400
• PubChem Substance ID: 24891468
• NACRES: NA.76
• Beilstein/REAXYS Number: 78342
• MDL number: MFCD00877763

คุณสมบัติ

• ชื่อสินค้า: Amphotericin B solubilized, powder, γ-irradiated, BioXtra, suitable for cell culture
• Quality Segment: 300
• sterility: γ-irradiated
• product line: BioXtra
• form: powder
• technique(s): cell culture | mammalian: suitable
• antibiotic activity spectrum: fungi, yeast
• mode of action: cell membrane | interferes
• storage temp.: 2-8°C
• SMILES string: C[C@H]1O[C@@H](O[C@@H]2C[C@@H]3O[C@](O)(C[C@@H](O)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)CC(=O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\2)C[C@H](O)[C@H]3C(O)=O)[C@@H](O)[C@@H](N)[C@@H]1O
• InChI: 1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
• InChI key: APKFDSVGJQXUKY-INPOYWNPSA-N

คำอธิบาย

General description

Chemical structure: polyene

Application

Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Biochem/physiol Actions

Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.

Packaging

Solubilized Amphotericin B is packaged in amber glass bottle.

Preparation Note

Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution.
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.

It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).

Other Notes

After reconstitution with water, the product will be a suspension of Amphotericin B, containing deoxycholate as the solubilizing agent. The lyophilized formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride.

Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.

The actual solid weight of the packaged product is calculated according to the UV purity.

Disclaimer

Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

ส่วนประกอบของชุดอุปกรณ์เท่านั้น

• Amphotericin B ~ 45 %
• Sodium deoxycholate ~ 35 %
• Sodium phosphate balance

ข้อมูลความปลอดภัย

• สัญลักษณ์รูปภาพ: GHS07
• คำเตือน: Warning
• รหัสอันตราย: H315,H319,H335
• จุดติดไฟ (°F): Not applicable
• จุดวาบไฟ (°C): Not applicable
• อุปกรณ์ป้องกันส่วนบุคคล (PPE): dust mask type N95 (US), Eyeshields, Gloves
• อวัยวะเป้าหมาย: Respiratory system
• คำเตือน: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• คลาสการจัดเก็บ: 11 - Combustible Solids