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Merck

A7638

Bovine Serum Albumin

lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

CAS Number:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:

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Product Name

Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

biological source

bovine

assay

≥99% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

~66 kDa

purified by

cold ethanol and heatshock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

blocking: suitable
microbiological culture: suitable

impurities

essentially globulin free

pH

7

solubility

water: soluble (50 mg/ml)

UniProt accession no.

storage temp.

2-8°C

Quality Level

Gene Information

bovine ... ALB(280717)

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This Item
A3156A9418A1470
origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

assay

≥99% (agarose gel electrophoresis)

assay

≥99% (agarose gel electrophoresis)

assay

≥96% (agarose gel electrophoresis)

assay

≥98% (agarose gel electrophoresis)

technique(s)

blocking: suitable, microbiological culture: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Purified by a combination of heat shock and ethanol fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Marcos V A S Navarro et al.
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