R7017

Resazurin sodium salt

powder, BioReagent

• Synonym(s): 7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt
• Empirical Formula (Hill Notation): C₁₂H₆NNaO₄
• CAS Number: 62758-13-8
• Molecular Weight: 251.17
• Beilstein: 3794971
• EC Number: 263-718-5
• MDL number: MFCD00005036
• UNSPSC Code: 12352207
• PubChem Substance ID: 24899387
• NACRES: NA.75

Properties

• product line: BioReagent
• form: powder
• composition: Dye content, ~80%
• pH: 3.8-6.5, orange to violet
• solubility: H₂O: soluble
• λmax: 600 nm
• SMILES string: [Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1
• InChI: 1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
• InChI key: IVGPGQSSDLDOLH-UHFFFAOYSA-M

Description

Application

For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).

Resazurin sodium salt has been used in viability assay:

• to measure cell viability of A. fumigatus[1][2]
• to assess cell proliferation[3]
• to determine A. fumigatus viability in high-throughput screenings of microbial natural extracts[4]

Biochem/physiol Actions

Resazurin is a blue, non-fluorescent dye originally used to test milk/biological fluids for bacterial and yeast contamination. It is also useful in sperm viability and semen quality test. Resazurin is applicable in cytotoxicity determination.[5]

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves