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Merck

RPROB

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
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usage

sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)

technique(s)

protein staining: suitable

storage temp.

2-8°C

Quality Level

Application

R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.

Other Notes

Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.

pictograms

Corrosion

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class

8B - Non-combustible corrosive hazardous materials


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Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mapping
Rachel R.
Electrophoresis (1997)

Articles

Sigma 提供 EZBlue™ 和 ProteoSilver™ 用於蛋白質可視化的試劑,適用於蛋白質組學和傳統 PAGE 格式。

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Protocols

Western 印迹法包括通过凝胶电泳分离蛋白质,将蛋白质转移到聚偏二氟乙烯 (PVDF) 或硝酸纤维素膜上,并通过各种方法进行检测。

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

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