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PROBES

Custom qPCR Probes

Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR

Synonym(s):
Black Hole Quencher, locked nucleic acid, qPCR designs, BHQ, Real-Time PCR Probes, Probes, BHQ Probes, qPCR assays, Locked Nucleic Acid probes


Dual-Labeled Probes

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

Molecular Beacons

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

LightCycler® Probes

  • Amounts: 0.1, 0.25, 1.5, and 15 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Probe 1 fluorescein (3′ end); Probe 2 phosphate (3′ end) and LC Red 610, 640, 670 or 705 (5′ end)
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

Scorpions® Probes

  • Amounts: 1, 5, and 5 OD
  • Purification: HPLC
  • Sequence Lengths: 30 - 60 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

Locked Nucleic Acid

  • Available for all Custom qPCR Probe Types
  • Increased thermal stability and hybridization specificity
  • Easier and more specific designs for problematic target sequences
  • Learn more

Black Hole Quencher® (BHQ®)

  • Available for all Custom qPCR Probe types
  • No native fluorescence (emits heat instead of light), resulting in lower background fluorescence
  • Increased signal-to-noise ratio, providing higher sensitivity
  • Enables a wider choice of reporters for multiplexing
  • Learn more
Ana F Almeida-Santos et al.
Scientific reports, 9(1), 473-473 (2019-01-27)
The absence of companion may jeopardize mental health in social animals. Here, we tested the hypothesis that social isolation impairs social recognition memory by altering the excitability and the dialog between the olfactory bulb (OB) and the dorsal hippocampus (dHIP)....
Rajeev Gupta et al.
Cell stem cell, 26(4), 527-541 (2020-03-21)
Umbilical cord blood (UCB) has had considerable impact in pediatric stem cell transplantation, but its wider use is limited in part by unit size. Long-term ex vivo culture offers one approach to increase engraftment capacity by seeking to expand stem and...
Articles
Two commonly used quenchers, TAMRA™ and DABYCL, limit the ultimate sensitivity and flexibility of qPCR. TAMRA is not a dark quencher and therefore contributes to an overall increase in background because of its own native fluorescence. DABCYL, though a dark quencher, has an inadequate absorption footprint that overlaps very poorly with reporter dyes emitting above 480 nm.
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Molecular Beacons are structured probes that are highly sensitive, sequence specific, and are used for sequence detection in qPCR and in vitro studies.
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Dual-Labeled Probes in Digital PCR
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Hudnall, et al. demonstrates the use of easy and effective qPCR assys to screen blood for herpesvirus.
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Related Content
LightCycler probes are highly sensitive and sequence-specific fluorescent probes designed for use with the Roche LightCycler instruments.
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Onxy Quencher emits heat instead of light and offers and improved signal-to-noise ratio over fluorescent quenchers.
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For more complex and demanding applications, utilize our consultative service to ensure the success of your assay.
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Label licenses for our oligonucleotide products, including Black Hole Quencher®, BlackBerry® Quencher, and Locked Nucleic Acid (LNA®).
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