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IPFL00005

Millipore

Immobilon®-FL PVDF Membrane

1 roll, 27 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

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Synonym(s):
Western blotting membrane, blotting membrane, transfer membrane
eCl@ss:
32031602
NACRES:
NB.23

material

PVDF membrane
plain filter
white filter

Quality Level

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

27 cm × 1.875 m

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin)
205 μg/cm2 adsorption capacity (BSA)
300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with All fluorescence dyes
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

ambient

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This Item
IPVH00005IPVH20200IPFL00010
feature

hydrophobic

feature

hydrophobic

feature

hydrophobic

feature

hydrophobic

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

dot blot: suitable, western blot: suitable

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

capacity

160 μg/cm2 (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

Application

Immobilon®-FL PVDF membrane has been used in western blotting.

Features and Benefits

Immobilon®-FL PVDF membrane is compatible with the following stains:
  • All fluorescence dyes
  • Coomassie brilliant blue
  • Amido black
  • Ponceau-S red
  • CPTS
  • Sypro® ruby

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
SYPRO is a registered trademark of Life Technologies

Storage Class Code

10-13 - German Storage Class 10 to 13


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Articles

The possible causes and potential remedies for poor or inconsistent transfer of proteins from a gel to the Western blot membrane. Optimal transfer conditions may vary depending on the molecular weight of the protein of interest.

Protocols

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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