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MilliporeSigma

IPFL00005

Immobilon® -FL PVDF Membrane

1 roll, 27 cm x 1.875 m, 0.45 µm pore size, transfer membrane with low background fluorescence

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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1 EA

$309.00

$309.00


Estimated to ship onDecember 23, 2025



About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.23

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Product Name

Immobilon®-FL PVDF Membrane, 1 roll, 27 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

material

PVDF membrane
plain filter
white filter

Quality Level

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

27 cm × 1.875 m

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin)
205 μg/cm2 adsorption capacity (BSA)
300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with All fluorescence dyes
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

ambient

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IPVH00005IPVH20200IPFL00010
material

PVDF membrane, white filter, plain filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

detection method

chemiluminescent, fluorometric, colorimetric

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

detection method

chemiluminescent, colorimetric, fluorometric

compatibility

for use with All fluorescence dyes, for use with CPTS, for use with Ponceau-S red, for use with Amido black, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Coomassie brilliant blue

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

compatibility

for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

Application

Immobilon®-FL PVDF membrane has been used in western blotting.

Features and Benefits

Immobilon®-FL PVDF membrane is compatible with the following stains:
  • All fluorescence dyes
  • Coomassie brilliant blue
  • Amido black
  • Ponceau-S red
  • CPTS
  • Sypro ruby

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.

Storage Class

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

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Articles

Troubleshoot protein transfer issues in Western blotting by understanding causes and optimizing transfer conditions.

Protocols

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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