A6778

Angiotensin Converting Enzyme from rabbit lung

≥2.0 units/mg protein (modified Warburg-Christian)

• Synonym(s): ACE, Peptidyl-dipeptidase A, Peptidyldipeptide Hydrolase
• CAS Number: 9015-82-1
• Enzyme Commission number: 3.4.15.1 (BRENDA, IUBMB)
• MDL number: MFCD00130325
• eCl@ss: 42010109
• NACRES: NA.32

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥2.0 units/mg protein (modified Warburg-Christian)
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The angiotensin-converting enzyme (ACE) is a dipeptidyl-carboxypeptidase which exists in somatic and testicular isoforms with zinc binding motif HEXXH in their active site. ACE regulates blood pressure through renin-angiotensin system. ACE elevates blood pressure by converting angiotensin I to a key vasoconstrictor angiotensin II and inhibiting a potent vasodilator bradykinin. Inhibition of ACE is a targeted therapeutic strategy for high blood pressure. Several ACE synthetic inhibitory peptides available for clinical use include captopril, enalapril and lisinopril. Currently, developing inhibitory peptides from natural food sources, or phenolic compounds from plant sources to inhibit ACE is underway. ACE plays a critical role in fertilization by releasing the proteins anchored to glycosylphosphatidylinositol (GPI) in sperm membrane.

Application

Angiotensin converting enzyme from rabbit lung has been used:

• for measuring inhibitory effect of egg white protein hydrolysates on ACE activity by high performance liquid chromatography (HPLC)
• to measure the ACE inhibition by litchi pericarp and cooked chicken breast using hippuryl-L-histidyl-L-leucine (HHL) as substrate by reverse phase-HPLC (RP-HPLC)3 and HPLC respectively
• in releasing GPI anchored protein in vitro in few cell lines like HeLa, HEK293 and in vivo in mice sperm.

Packaging

0.1, 0.25, 1 unit

2, 5 units

Biochem/physiol Actions

Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin.

Quality

May contain traces of sodium chloride.

Unit Definition

One unit will produce 1.0 μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves