The milligram quantity of this product will vary from lot to lot. The vial content is dependent upon the protein content and enzymatic activity. The minimum protein content will be 70% and the minimum activity will be 2 units per milligram protein. As an example, a batch that reports a protein content of 100% and an activity of 2 units per milligram protein will have a vial content of 2.5 mg for the 5 unit package size. The protein content and activity are reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a6778#product-documentation
A6778
Angiotensin Converting Enzyme from rabbit lung
≥2.0 units/mg protein (modified Warburg-Christian)
Synonym(s):
ACE, Peptidyl-dipeptidase A, Peptidyldipeptide Hydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
42010109
NACRES:
NA.32
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form
lyophilized powder
Quality Level
specific activity
≥2.0 units/mg protein (modified Warburg-Christian)
shipped in
dry ice
storage temp.
−20°C
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This Item | A2580 | SAE0075 | 176872 |
|---|---|---|---|
| specific activity ≥2.0 units/mg protein (modified Warburg-Christian) | specific activity ≥10 units/mg protein (Bradford) | specific activity ≥10 U/mg | specific activity ≥0.8 units/μg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form liquid |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
General description
The angiotensin-converting enzyme (ACE) is a dipeptidyl-carboxypeptidase which exists in somatic and testicular isoforms with zinc binding motif HEXXH in their active site.[1] ACE regulates blood pressure through renin-angiotensin system. ACE elevates blood pressure by converting angiotensin I to a key vasoconstrictor angiotensin II and inhibiting a potent vasodilator bradykinin. Inhibition of ACE is a targeted therapeutic strategy for high blood pressure.[2][3] Several ACE synthetic inhibitory peptides available for clinical use include captopril, enalapril and lisinopril.[2] Currently, developing inhibitory peptides from natural food sources,[2][3][4] or phenolic compounds from plant sources to inhibit ACE is underway.[5] ACE plays a critical role in fertilization by releasing the proteins anchored to glycosylphosphatidylinositol (GPI) in sperm membrane.[1]
Application
Angiotensin converting enzyme from rabbit lung has been used:
- for measuring inhibitory effect of egg white protein hydrolysates on ACE activity by high performance liquid chromatography (HPLC)[2]
- to measure the ACE inhibition by litchi pericarp[5] and cooked chicken breast[4] using hippuryl-L-histidyl-L-leucine (HHL) as substrate by reverse phase-HPLC (RP-HPLC)3 and HPLC[4] respectively
- in releasing GPI anchored protein in vitro in few cell lines like HeLa, HEK293 and in vivo in mice sperm.[1]
Biochem/physiol Actions
Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin.
Quality
May contain traces of sodium chloride.
Unit Definition
One unit will produce 1.0 μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How many grams per 1 U?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Are there any other known inhibitors of rabbit lung Angiotensin Converting Enzyme (ACE), Product A6778?
1 answer-
Idrapril, the prototype of a new class of angiotensin converting enzyme (ACE) inhibitors, competitively inhibits, with nanomolar apparent Ki, the hydrolysis of hippuryl-glycyl-glycine by rabbit lung ACE. Kinetic Ki and kon and koff values were 470 pM, 3.0±1.5×106 M-1sec-1 and 1.4±0.3×10-3 sec-1, respectively. Reference: Biochemical Pharmacology, 45(6), 1358-1362 (1993).
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What is the effect of bleomycin on Angiotensin Converting Enzyme (ACE) activity in vivo?
1 answer-
Bleomycin causes a marked reduction in rabbit lung ACE activity. Reference: Cancer Research, 40, 3621-3626, (1980).
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What is the medical significance of Angiotensin Converting Enzyme (ACE), Product A6778?
1 answer-
Angiotensin-converting enzyme (ACE) produces angiotensin II, a substance that affects the cardiovascular system in numerous ways, including narrowing of blood vessels. This narrowing can cause high blood pressure and cause the heart to work harder. ACE inhibitors treat a variety of conditions, such as high blood pressure, scleroderma and migraines. (Mayo Clinic)
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