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C8696

Sigma-Aldrich

Cathepsin D from human liver

lyophilized powder, ≥250 units/mg protein (E1%/280)

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CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.32

form

lyophilized powder

Quality Level

specific activity

≥250 units/mg protein (E1%/280)

mol wt

~45 kDa

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... CTSD(1509)

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This Item
C3138SRP6415C8571
Cathepsin D human recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Sigma-Aldrich

SRP6415

Cathepsin D human

specific activity

≥250 units/mg protein (E1%/280)

specific activity

≥2.0 units/mg protein

specific activity

-

specific activity

≥1,500 units/mg protein (E1%/280)

mol wt

~45 kDa

mol wt

~45 kDa

mol wt

calculated mol wt 43.6 kDa, observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.)

mol wt

-

color

white

color

-

color

-

color

-

UniProt accession no.

P07339

UniProt accession no.

P80209

UniProt accession no.

P07339

UniProt accession no.

P07858

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

General description

Cathepsin D is an aspartic protease, which is located in lysosomes. It is involved in protein catabolism and maintains hormone and antigen processing. Cathepsin D is implicated in neoplasia and neurodegenerative changes. It regulates lysosomal proteolysis and endogenous fibrinolysis.

Application

Cathepsin D from human liver has been used:
  • in β-secretase activity assay
  • for enzymatic degradation of amyloid β 1-42
  • in microinjection of human foreskin fibroblasts

Biochem/physiol Actions

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Other Notes

Contains α, β and γ isoenzymes as observed by isoelectric-focusing.

Unit Definition

One unit will produce an increase in A280 of 1.0 in 30 min at pH 3.3 at 37 °C measured as TCA-soluble products using acid-denatured hemoglobin as substrate (1 cm light path).

Physical form

Powder containing sodium phosphate buffer salt.

related product

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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