NONH for all modes of transport
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Type IIA Cas9 derived from Streptococcus pyogenes.
In the monocot CRISPR Plant vectors, the Cas9 codon optimization is based on Zea mays. In the dicot CRISPR Plant vectors, the Cas9 codon optimization is based on Arabidopsis thaliana.
The promoter used is 2X35S, derived from cauliflower mosaic virus (CaMV).
The 2X35S promoter is used in this case.
In the monocot CRISPR vectors, it is a wheat U6 promoter. In the dicot CRISPR vectors, it is an Arabidopsis thaliana U6 promoter.
Yes. However, the biolistics/protoplast CRISPR vectors cannot be used for Agrobacterium-mediated plant transformation.
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