MilliporeSigma
All Photos(2)

D2158

Sigma-Aldrich

Histodenz

nonionic density gradient medium

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
5-(N-2,3-Dihydroxypropylacetamido)-2,4,6-triiodo-N,N′-bis(2,3-dihydroxypropyl)isophthalamide
Empirical Formula (Hill Notation):
C19H26I3N3O9
CAS Number:
Molecular Weight:
821.14
Beilstein:
2406632
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

form

powder

application(s)

hematology
histology

storage temp.

room temp

SMILES string

CC(=O)N(CC(O)CO)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I

InChI

1S/C19H26I3N3O9/c1-8(29)25(4-11(32)7-28)17-15(21)12(18(33)23-2-9(30)5-26)14(20)13(16(17)22)19(34)24-3-10(31)6-27/h9-11,26-28,30-32H,2-7H2,1H3,(H,23,33)(H,24,34)

InChI key

NTHXOOBQLCIOLC-UHFFFAOYSA-N

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
PHR193174147Y0000672
Histodenz™ nonionic density gradient medium

Sigma-Aldrich

D2158

Histodenz

Iohexol Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1931

Iohexol

Iohexol analytical standard

Supelco

74147

Iohexol

Iohexol for peak identification European Pharmacopoeia (EP) Reference Standard

Y0000672

Iohexol for peak identification

storage temp.

room temp

storage temp.

2-30°C

storage temp.

-

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

-

application(s)

hematology
histology

application(s)

pharmaceutical

application(s)

clinical testing

application(s)

pharmaceutical (small molecule)

General description

HistoDenz, also known as iohexol, is a non-ionic X-ray contrast compound developed specifically as an iodinated density gradient medium.

Application

HistoDenz is suitable for separating everything from macromolecules to viable cells by either rate-zonal or isopycnic centrifugation.
  • It has been used in the isolation and culturing of hepatic stellate cells.
  • It has also been employed in the extraction of microbial cells from environmental samples.
  • It has been used in LacZ (β-galactosidase) staining.
  • It has also been used for the enrichment of gonocytes.

Features and Benefits

HistoDenz offers several favorable properties as a gradient medium.
  • It has very low toxicity, low osmolality, high density, and low viscosity (compared to sucrose).
  • It is water-soluble, non-ionic, and chemically near-inert.

Legal Information

Histodenz is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

1-Thioglycerol ≥97%

Sigma-Aldrich

M1753

1-Thioglycerol

Meglumine diatrizoate organic

Sigma-Aldrich

M5266

Meglumine diatrizoate

Iodixanol European Pharmacopoeia (EP) Reference Standard

Y0001294

Iodixanol

Iodixanol United States Pharmacopeia (USP) Reference Standard

USP

1343517

Iodixanol

SnapShot: Tissue Clearing
Douglas S. Richardson, Jeff W. Lichtman
Cell, 171 (2017)
Skeletal muscle tissue clearing for lacz and fluorescent reporters, and immunofluorescence staining.
Verma M, et al.
Methods in Molecular Biology, 129-140 (2016)
Isolation and Culture of Hepatic Stellate Cells
Ralf Weiskirchen, Axel M. Gressner
Methods in Molecular Medicine, 117 (2005)
Extraction of Microbial Cells from Environmental Samples for FISH Approaches
Jennifer Pratscher
Methods in Molecular Biology, 2246 (2021)
Attachment of Pseudomonas putida onto differently structured kaolinite minerals: A combined ATR-FTIR and 1H NMR study.
Vasiliadou I A, et al.
Colloids and Surfaces. B, Biointerfaces, 84(2), 354-359 (2011)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service