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E2129

Sigma-Aldrich

Evans Blue

Dye content ≥75 %

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Synonym(s):
Direct Blue 53
Empirical Formula (Hill Notation):
C34H24N6Na4O14S4
CAS Number:
Molecular Weight:
960.81
Colour Index Number:
23860
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

composition

Dye content, ≥75%

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1\N=N\c2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(\N=N\c5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;

InChI key

ATNOAWAQFYGAOY-GPTZEZBUSA-J

Gene Information

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1 of 4

This Item
B0149302643C8679
Evans Blue Dye content ≥75 %

Sigma-Aldrich

E2129

Evans Blue

Brilliant Blue R Dye content ~50 %, Technical grade

Sigma-Aldrich

B0149

Brilliant Blue R

Trypan Blue Dye content 60 %

Sigma-Aldrich

302643

Trypan Blue

Chicago Sky Blue 6B powder

Sigma-Aldrich

C8679

Chicago Sky Blue 6B

composition

Dye content, ≥75%

composition

Dye content, ~50%

composition

Dye content, 60%

composition

Dye content, ~80%

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Gene Information

human ... PTPN1(5770), PTPRF(5792)

Gene Information

-

Gene Information

human ... PTPN1(5770), PTPRF(5792)

Gene Information

-

General description

Evans blue (EB) C.I. 23860 is a synthetic bis-azo dye also known as T-1824 and Direct Blue 53. EB dye with higher water solubility has a strong affinity towards serum albumin, making it a high molecular weight protein tracer.

Application

Evans blue is a well-known vital stain with applications in histology and fluorescent microscopy. Evans blue has been used to check cell viability and study blood-brain barrier (BBB) permeability. Additional applications include the demonstration of specific pituitary cell types, selective staining of neutrophil plasma membranes, and fluorescence microscopy as a quenching agent to suppress unwanted autofluorescence.

Biochem/physiol Actions

Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Methods in molecular biology (Clifton, N.J.), 686, 371-378 (2010-11-18)
The blood-brain barrier (BBB) is a monolayer of endothelial cells that is regulated by the proximity of a unique basement membrane and a tightly controlled molecular interaction between specialized subsets of cells including pericytes, astrocytes, and neurons. Working together, these
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CO2 is the main inorganic C species entering photosynthetically active leaf protoplasts of the freshwater macrophyte Ranunculus penicillatus ssp. pseudofluitans.
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Canonical WNT signaling is required for proper vascularization of the CNS during embryonic development. Here, we used mice with targeted mutations in genes encoding canonical WNT pathway members to evaluate the exact contribution of these components in CNS vascular development

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