M2003

Sigma-Aldrich

MTT Formazan

powder

Synonym(s):
1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan
Empirical Formula (Hill Notation):
C18H17N5S
CAS Number:
Molecular Weight:
335.43
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥97% (TLC)

form

powder

application(s)

hematology: suitable
histology: suitable

color

purple

solubility

acetone: 1 mg/mL

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI key

GIFKVQOPAJDJLO-SZVLODPRSA-N

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Application

MTT Formazan has been used to measure cell viability.

Packaging

1 g in glass bottle

Biochem/physiol Actions

MTT method is suitable to check cell viability during apoptosis. Live cells reduce MTT (yellow colored) to formazan MTT (dark blue product). The presence of formazan MTT can be measured spectrophotometrically at 570nm.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can MTT Formazan, Product M2003, be used in cell viability assays?

    No.  MTT or thiazolyl blue tetrazolium is used to determine the number of viable cells in well-cultures. Consider use of kit CGD1 or product M5655.

  4. What is the solubility of MTT Formazan, Product M2003, in water or PBS?

    This product is not soluble in either water or PBS.  Sigma Aldrich tests product at 1mg/mL in acetone. A 1:1 DMSO:acidified isopropanol also works well for dissolving the formazan.

  5. Can MTT Formazan, Product M2003, be used to measure dehydrogenases?

    No. MTT is converted by active mitochondrial dehydrogenases of living cells to MTT Formazan. Consider use of product M2128 or M5655.

  6. What is the proper wavelength for measuring MTT Formazan, Product M2003?

    We recommend detection at 570 nm, with background subtraction at 630-690 nm in acidified isopropanol.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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