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Packaging
1 kit
Application
The GlycoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.
Principle
The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.
Quantity
Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.
Legal Information
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
- Acetonitrile, ≥99.93%, BioSyn® 50 mL
Kit Components Also Available Separately
Product No.
Description
SDS
- P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 USDS
- T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μgSDS
- Trypsin Solubilization Reagent
- I0408Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiae, for proteomicsSDS
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point(F)
35.6 °F
Flash Point(C)
2.0 °C
Certificate of Analysis
Enter Lot Number to search for Certificate of Analysis (COA).
Certificate of Origin
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Product Information Sheet
Newsletter / Publication
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