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PP0200

Sigma-Aldrich

GlycoProfile I, In-Gel Deglycosylation Kit

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EC Number:
NACRES:
NA.32

shelf life

≥1 yr at 2‑8 °C

Quality Level

storage temp.

2-8°C

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PP0510124013CC172
Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

shelf life

≥1 yr at 2‑8 °C

shelf life

-

shelf life

-

shelf life

-

Application

The GlycoProfile Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.

Principle

The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.

Quantity

Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description

  • Acetonitrile, ≥99.93%, BioSyn® 50 mL

Kit Components Also Available Separately

Product No.
Description
SDS

  • P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 USDS

  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μgSDS

  • Trypsin Solubilization Reagent

  • I0408Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiae, for proteomicsSDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

35.6 °F

Flash Point(C)

2.0 °C


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25G
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705578-5MG-PW

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Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Deglycosylation Kits

Deglycosylation Kits

O-Linked Glycan Strategies

Learn about O-linked glycan strategies, such as the actions of O-glycosidase, how to remove di and trisialylation sialic acid residues, β-linked galactose, and N-acetylglucosamine, as well as other O-glycan modifications.

Strategies for Deglycosylating N-Linked Glycans

Explore various strategies for deglycosylating N-linked glycans involving PNGase F, PNGase A (Glycopeptidase A), and even native and sequential deglycosylation with endoglycosidases like Endoglycosidase H, Endoglycosidase F, and exoglycosidases.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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