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TP0010

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FLAG® HA Tandem Affinity Purification Kit

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Synonym(s):
Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

storage temp.

2-8°C

Quality Level

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FLAGIPT1F2426A2220
FLAG® Immunoprecipitation Kit

FLAGIPT1

FLAG® Immunoprecipitation Kit

Millipore

A2220

ANTI-FLAG® M2 Affinity Gel

General description

TAP technology is a recent development in protein purification. This technology incorporates tandem-linked affinity tags into genes of interest so that high purity fusion proteins can be isolated through two consecutive affinity purification steps. The technology is particularly useful for the isolation and identification of protein complexes by adding a tandem affinity tag to a known "bait" protein to pull down endogenous proteins that interact with the targeted protein of interest.

Application

The FLAG® HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and tissue homogenates. The kit is particularly suitable for isolation of protein complexes using TAP (Tandem Affinity Purification) technology.

Sutitable for mass spectrometry analysis and western blotting.

Learn more product details in our FLAG® application portal.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS

  • F2426EZview Red ANTI-FLAG® M2 Affinity Gel, clone M2SDS

  • A2095Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspensionSDS

  • R0278RIPA BufferSDS

  • F47993X FLAG® Peptide, lyophilized powderSDS

  • U4883Urea, 8 M (after reconstitution with 16 mL high purity water)SDS

pictograms

Exclamation markEnvironment

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2

Storage Class

10 - Combustible liquids


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Articles

The FLAG® HA tandem epitope system consists of small epitopes tags that are not eukaryotic derived. These features minimize interference with protein functions and provide superior specificity.

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