TSTOP

ThermaStop^™

increases PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before PCR, improves hot start PCR

• Synonym(s): Multiplex PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart
• UNSPSC Code: 12352200
• eCl@ss: 32150313

Properties

• form: lyophilized powder
• packaging: pkg of 2500 units, pkg of 500 units, pkg of One unit of ThermaStop^™ is defined as the amount required for maximum hot-start in amplification reactions containing one unit of Taq DNA polymerase in a volume of 25 μl.
• technique(s): PCR: suitable, whole genome amplification: suitable
• color: opaque
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

ThermaStop^™ is a reversible hot-start reagent that is compatible with thermostable DNA polymerases. It acts directly on the polymerase to prevent non-specific enzymatic activity below 50°C. Polymerase activity is fully restored at 60°C, but is inhibited again by ThermaStop^™ by cooling the reaction. Adding ThermaStop^™ to your PCR reactions improves sensitivity and total product yield.

Application

ThermaStop^™ has been used as a component of the PCR mix for the human immunodeficiency virus (HIV-1) proviral genome amplification by non-multiplexed nested PCR. It has also been used for the amplification of DNA from oral swabs of HIV infected individuals.

Features and Benefits

• Adding ThermaStop^™ to your PCR reactions improves sensitivity and total product yield
• Increases the product yield for downstream applications like genotyping, next-generation sequencing (NGS) sample preparation, Gibson assembly, diagnostics, and multiplex PCR
• It improves polymerase specificity, enhances PCR performance and quantitative data
• Prevents primer-dimer formation and non-specific products before PCR
• Enables stabilization of master mixes at room temperature before PCR for up to 36 hrs
• Rapidly inactivated during the first PCR cycle
• Rapidly re-activated upon cooling

Preparation Note

Dissolve in Tris pH 8.3 buffer

Legal Information

ThermaStop is a trademark of ThermaGenix Inc.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable