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TSTOP

Sigma-Aldrich

ThermaStop

increases PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before PCR, improves hot start PCR

Synonym(s):
hot start PCR, hotstart, Multiplex PCR, PCR Additive, hot start DNA polymerase

assay

(Dual, HPLC)

form

lyophilized powder

packaging

pkg of 2500 units
pkg of 500 units
pkg of One unit of ThermaStop is defined as the amount required for maximum hot-start in amplification reactions containing one unit of Taq DNA polymerase in a volume of 25 μl.

technique(s)

PCR: suitable

color

opaque

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

ThermaStop is a reversible hot-start reagent that is compatible with thermostable DNA polymerases. It acts directly on the polymerase to prevent non-specific enzymatic activity below 50°C. Polymerase activity is fully restored at 60°C, but is inhibited again by ThermaStop by cooling the reaction. Adding ThermaStop to your PCR reactions improves sensitivity and total product yield.

Application

ThermaStop has been used as a component of the PCR mix for the human immunodeficiency virus (HIV-1) proviral genome amplification by non-multiplexed nested PCR. It has also been used for the amplification of DNA from oral swabs of HIV infected individuals.

Features and Benefits

  • Adding ThermaStop to your PCR reactions improves sensitivity and total product yield
  • Increases the product yield for downstream applications like genotyping, next-generation sequencing (NGS) sample preparation, Gibson assembly, diagnostics, and multiplex PCR
  • It improves polymerase specificity, enhances PCR performance and quantitative data
  • Prevents primer-dimer formation and non-specific products before PCR
  • Enables stabilization of master mixes at room temperature before PCR for up to 36 hrs
  • Rapidly inactivated during the first PCR cycle
  • Rapidly re-activated upon cooling

Reconstitution

Dissolve in Tris pH 8.3 buffer

Legal Information

ThermaStop is a trademark of ThermaGenix Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Articles

ThermaStop™-RT PCR Additive

PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.

ThermaGenix Product Overview

How to stop nonspecific bands and off target primers in PCR.

ThermaStop™ PCR Additive

PCR additive added to master mix that stops nonspecific bands after cool down.

Protocols

Therma™ PCR Additive

PCR additive for the annealing and extension steps that reduce scatter and increase product signals.

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