Chemical incorporation of 1-methyladenosine into oligonucleotides.

Chemical incorporation of 1-methyladenosine into oligonucleotides.

Nucleic acids research (2002-02-28)

Sergey N Mikhailov, Jef Rozenski, Ekaterina V Efimtseva, Roger Busson, Arthur Van Aerschot, Piet Herdewijn

PMID11861902

RESUMEN

The base moiety of 1-N-methyladenosine can be protected with a chloroacetyl group for incorporation of this modified nucleoside into DNA and RNA. Carefully controlled anhydrous conditions are needed for deprotection of the oligonucleotides.