[Gene expression analysis by DNA microarray in papillary thyroid cancer].

[Gene expression analysis by DNA microarray in papillary thyroid cancer].

Endokrynologia Polska (2006-07-04)

Elzbieta Gubała, Małgorzata Wiench, Małgorzata Oczko-Wojciechowska, Dariusz Lange, Jan Włoch, Agnieszka Czarniecka, Ewa Chmielik, Barbara Nikiel, Zbigniew Wygoda, Aleksandra Kukulska, Daria Handkiewicz-Junak, Sylwia Szpak-Ulczok, Aleksandra Krawczyk, Józef Roskosz, Barbara Jarzab

PMID16817140

RESUMEN

In our study we present chosen elements of microarray analysis of gene expression profile in papillary thyroid cancer. The study group included 16 papillary thyroid cancer tissues and 16 corresponding normal tissues. Samples were analyzed on high density oligonucleotide microarrays (GeneChip HG-U133A) which contain 22.000 genes. 110 genes, which had significant changed expression, were selected by MAS 5.0 program. 3 genes were chosen to the deeper analysis: dipeptidylpeptidase 4 (DPP4), fibronectin 1 (FN1), tissue inhibitor of metalloproteinase 1 (TIMP1). DPP4-RNA were absent in normal tissue while in cancer tissue it was detected in large amount. FN1 and TIMP1 expression were detected in normal tissue but markedly increased in papillary thyroid cancer. Among these 3 genes DPP4 seems to be the best molecular marker for papillary thyroid cancer.

MATERIALES

Número de producto

Marca

Descripción del producto

F5007

Sigma-Aldrich

Fibronectin Type III Connecting Segment Fragment 1-25, ≥90% (HPLC)