This product is not purified but supplied as culture supernatant, the concentration is not determined. However, typical concentration range for antibody supplied as a culture supernatant would be 25-125 µg/ml.
04-745
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal
culture supernatant, clone MC315, Upstate®
Synonym(s):
H3K4me3, Histone H3 (tri methyl K4)
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100 μL
R 9,826.46
R 9,826.46
List PriceR 10,027.00Please contact Customer Service for Availability
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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Product Name
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal, culture supernatant, clone MC315, Upstate®
biological source
rabbit
antibody form
culture supernatant
antibody product type
primary antibodies
clone
MC315, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
multiplexing: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
trimethylation (Lys4)
Quality Level
Gene Information
human ... H3C1(8350)
1 of 4
This Item | 05-808 | 05-1248 | 05-1951 |
|---|---|---|---|
| biological source rabbit | biological source rabbit | biological source mouse | biological source mouse |
| species reactivity human | species reactivity human | species reactivity human | species reactivity human, mouse |
| Gene Information human ... H3C1(8350) | Gene Information human ... H3C1(8350) | Gene Information human ... H3C1(8350) | Gene Information human ... H3C1(8350) |
| clone MC315, monoclonal | clone monoclonal | clone CMA306, monoclonal | clone 18E9.1, monoclonal |
| antibody form culture supernatant | antibody form purified antibody | antibody form purified immunoglobulin | antibody form - |
| isotype IgG | isotype IgG | isotype IgG2a | isotype IgG2bκ |
Application
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315 is a rabbit monoclonal antibody for detection of trimethyl-Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in WB, ChIP, DB, Mplex, ChIP-seq.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in human colon and human breast cancer tissue sections
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in HeLa, A431, HUVEC, and NIH3T3 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in HeLa, A431, HUVEC, and NIH3T3 cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Analysis Note
routinely evaluated by immunoblot on in acid extracted proteins from HeLa cells, but not recombinant unmethylated Histone H3 (Catalog #14-494)
Control
HeLa acid extract
HeLa acid extract
Biochem/physiol Actions
Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See COA for specificity data.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
17 kDa
Immunogen
Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
Other Notes
Replaces: 04-791
Physical form
Cultured supernantant in 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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PLoS pathogens, 7(2), e1001292-e1001292 (2011-03-08)
Plasmodium falciparum employs antigenic variation to evade the human immune response by switching the expression of different variant surface antigens encoded by the var gene family. Epigenetic mechanisms including histone modifications and sub-nuclear compartmentalization contribute to transcriptional regulation in the
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What is the concentration?
1 answer-
This product is provided as a cell culture supernatant. Unfortunately. the specific antibody concentrations is not determined. However, the typical expected range is 25-125 µg/mL.
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