ABE1025

Anti-Achaete-scute homolog 1 Antibody (ASCL1)

from rabbit, purified by affinity chromatography

• Synonym(s): Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human
• technique(s): immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_004307
• UniProt accession no.: P50553
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ASCL1(429)

Description

General description

Achaete-scute homolog 1 (ASCL1), also known as MASH1, ASH1 or HASH1, is a basic helix-loop-helix transcription factor. ASCL1 binds to the E box (5′-CANNTG-3′) which then activates transcription. For efficient DNA binding, dimerization with other BHLH proteins is required. Achaete Scute proteins may also be involved in the determination of neuronal precursors in the central and peripheral nervous systems. It is expressed in fetal brain and is essential for the proper development and the survival of subsets of autonomic neurons. It is highly expressed in medullary thyroid cancer and small cell lung cancer.

~38 kDa observed

Immunogen

BCP-conjugated linear peptide corresponding to human Achaete-scute homolog 1 (ASCL1).

Application

This Anti-Achaete-scute homolog 1 Antibody (ASCL1) is validated for use in Western Blotting and Immunohistochemistry for the detection of Achaete-scute homolog 1 (ASCL1).

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Achaete-scute homolog 1 (ASCL1) in 5 µg of mouse E13-14 brain lysate and A549 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Achaete-scute homolog 1 (ASCL1) in rat cerebellum, rat cerebral cortex, and rat hippocampus tissue.

Analysis Note

Evaluated by Western Blotting using lysate from cells over expressing Achaete-scute homolog 1 (ASCL1).

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Achaete-scute homolog 1 (ASCL1) in 5 µg of lysate from cells over expressing Achaete-scute homolog 1 (ASCL1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable