FCMAB113A4

Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor^™ 488 conjugate

clone 10H11.2, from mouse, ALEXA FLUOR^™ 488

• Synonym(s): Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 10H11.2, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable
• isotype: IgG1
• NCBI accession no.: NM_002701.4
• UniProt accession no.: Q01860
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... POU5F1(5460)

Description

General description

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

~44 kDa

Immunogen

Recombinant GST fusion protein, human Oct-4 (aa 1-134).

Application

Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.

Flow Cytometry:
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments

Working Solution	Cells / test	Total Reaction Volume
5 μl	5 x 105 Cells / 95 μl PBS	100 μl
10 μl	1 x 106 Cells / 90 μl PBS	100 μl

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Biochem/physiol Actions

Reacts with human Oct-4.

Physical form

Purified mouse monoclonal IgG1 conjugated to Alexa Flour 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.

Preparation Note

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt

Analysis Note

Control
Human embryonic stem cells

Evaluated by Flow Cytometry in 2102Ep Cells.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable